Cell_Adhesion_protocol_manual[1].pdfVIP免费

BDBiosciencesCellAdhesionProtocolManualBDBiosciencesForResearchUseOnly.Notforuseindiagnosticortherapeuticprocedures.Purchasedoesnotincludeorcarryanyrighttoresellortransferthisproducteitherasastand-aloneproductorasacomponentofanotherproduct.AnyuseofthisproductotherthanthepermittedusewithouttheexpresswrittenauthorizationofBectonDickinsonandCompanyisstrictlyprohibited.BD,BDLogoandallothertrademarksarethepropertyofBecton,DickinsonandCompany.©2005BDFicollisatrademarkofAmershamBiosciencesABHypaqueisatrademarkofAmershamHealthAS©2005Becton,DickinsonandCompany.Allrightsreserved.Nopartofthispublicationmaybereproduced,transmitted,transcribed,storedinretrievalsystems,ortranslatedintoanylanguageorcomputerlanguage,inanyformorbyanymeans:electronic,mechanical,magnetic,optical,chemical,manual,orotherwise,withoutpriorwrittenpermissionfromBDBiosciences.CellAdhesion1STEditionSeptember2001ProtocolManualTableofContentsIntroduction...................................1-2TechniquesICellCulture.......................................2IICellCultureProductList...........................3-4IIIAnalysisofCellAdhesionMoleculeExpressionA.FlowCytometricAnalysis.......................5-7B.ImmunohistochemicalStaining...................7-9C.ImmunofluorescentLabelingofCellsinCulture....10-11D.Immunoblotting............................11-15E.BDRiboQuant™AssayandProductList..........15-22IVQuantitationofCellAdhesiontoImmobilizedLigandA.ColorimetricCellAdhesionAssays..............23-26B.RadiometricDeterminationofCellAdhesion......27-28C.Fluorescence-basedCellAdhesionAssay.........28-31D.ComparisonofAssayMethods.................31-33E.QuantitationofCell-CellAdhesion................33VAntibodyProductList..........................33-52VIReferences......................................53www.bdbiosciences.com1IntroductionAdhesionmoleculesenablecellstocontactandspecificallyinteractwitheachother,arequirementofallmulticellularorganisms.Specializedcell-cellinteractionsallowcommunicationbetweenadjacentcellsandar...