基金项目:上海市浦东新区卫生系统重点学科建设资助项目(PWZXK2017-09)通信作者:郭建Correspondenceto:GUOJianE-mail:guojian1110@126.comdoi:10.3969/j.issn.1673-6184.2023.02.005·病例分析·空肠弯曲杆菌血流感染报道1例熊丽娟1,郭建21.贵州中医药大学第二附属医院检验科,贵州贵阳550001;2.上海市东方医院南院检验科,上海200120摘要:本文报道了空肠弯曲杆菌血流感染1例,并研究了其生物学特性与可能存在的耐药基因,为临床有效治疗相关疾病提供科学的实验室依据。采用常规方法、基质辅助激光解吸电离飞行时间质谱分析、测序分析和聚合酶链反应(polymerasechainreaction,PCR)等技术,对从1位老年女性血液中分离出的1株致病菌进行菌种鉴定、药物敏感试验、耐药基因检测等。结果显示,此分离菌株为空肠弯曲杆菌,其对环丙沙星、四环素耐药,对红霉素敏感。采用PCR检测出空肠弯曲杆菌的3种耐药基因gyrA、gyrB、tex(O),可能与其药敏试验结果相关。关键词:空肠弯曲杆菌;血流感染;耐药基因中图分类号:R372;R378文献标识码:AAcaseofbloodstreaminfectioncausedbyCampylobacterjejuniXIONGLijuan1,GUOJian21.DepartmentofLaboratoryMedicine,theSecondAffiliatedHospitalofGuizhouUniversityofTraditionalChineseMedicine,Guiyang550001,GuizhouProvince,China;2.DepartmentofLaboratoryMedicine,ShanghaiEastHospital,TongjiUniversitySchoolofMedicine,Shanghai200120,ChinaAbstract:AcaseofbloodstreaminfectioncausedbyCampylobacterjejuniwasreported,anditsbiologicalcharacteristicsandpossibledrugresistancegeneswasstudiedtoprovidescientificlaboratorybasisforeffectiveclinicaltreatmentofrelateddiseases.Thestrainisolatedfromthebloodofanelderlyfemalewasidentifiedbyconventionalmethods,matrix-assistedlaserdesorptionionizationtimeofflightmassspectrometry(MALDI-TOFMS),sequencingandpolymerasechainreaction(PCR).ResultsshowthattheisolatedstrainwasCampylobacterjejuni,anditwasresistanttociprofloxacinandtetracyclineandsensitivetoerythromycin.ThreedrugresistancegenesgyrA,gyrBandtex(O)inCampylobacterjejuniweredetectedbyPCR,whichmayberelatedtotheresultsofdrugsensitivitytest.Keywords:Campylobacterjejuni;Bloodinfection;Drugresistancegene弯曲杆菌属是一种呈弯曲状、“S”形或螺旋状的杆菌,菌体直径为0.2~0.9μm...
