1TitlepageXXXattenuateXXX‐inducedXXXdiseasethrough/bytheinhibition/regulationofXXXRunningtitle:XXXattenuateXXXdiseaseXiao‐mingWang1,SanZhang2,ChongLing‐hu1*1departmentofXXX,XXXuniversity,XXXcity123456,China.2departmentofXXX,XXXuniversity,XXXcity123456,China.*Correspondenceto:ProfessorChongLing‐hu,M.D.,Ph.D.DepartmentofClinicalMedicine,XXXUniversityCityCollegeSchoolofMedicine000XXXStreet,XXXcity123456,P.R.ChinaEmail:XXX@163.com2AbstractBackgroundRationalforstudyandgapinknowledge(1‐2sentences)Hypothesisorpurposestatement(1sentence)Generalexperimentaldesign(optional)Methods(Oneresultwithonemethod.)XXXwasdetermined/detected/measured/evaluated/examinedbyXXXmethod/experiment.Wedetermined/detected/measured/evaluated/examinedtheXXXlevelusingXXXmethod/experiment.Ect.ResultsWefonndthat….Comparedwith….group,XXXwasincreased/decreased/enhanced/reducedbyXXXtreatment/administrations.ConclusionsandimplicationsTheseresults/dataweresuggested/indicated/demonstratedthat…..Keyword:5‐6keywords.3IntroductionBackground:Known(Generaltospecific)Unknown:gap,howstudywillfillgap,andwhyfillinggapisimportantUntilnow,ithasnotbeenpossibletoidentify….However,wedonotknowwhateffectthistreatmentwillhaveon….Therehasbeensomedisagreement,however,inthefindingswiththismodel….Sofar,onlyqualitativefindingshavebeenobtained…Becauseoftherarityofthiscancer,ithasbeendifficulttodeterminesurvivalrateswithstatisticalcertainty…HypothesisThepurposeofourstudywastodetermine….Wehypothesizedthat….Ourhypothesiswasthat….StrategyWetestedourhypothesisbyexamining….Todothis,weused…andanalyzed…inansitumodel….4Toevaluate/investigate/exploretheinhibitory/regulatoryeffectof…on….,the….Assay/methods/experimentswereemployed/used.ConclusionOverall,ourfindingsthat…confirm/indicate/suggest/demonstrate….Inthisstudy,wefound/observed/demonstratedthat….Inthepresentstudy,wefound/observed/demonstratedthat….5MaterialsandmethodsMaterialsandreagentsDrugs,reagents,equipment,antibodies,ect.CelllinesandculturesWhicho...