技术与应用生命科学仪器2023第21卷/1期50Abstract:Thecurrentfluorescenceimmunochromatographyislessaccurateinfindingtestpeakforlowconcentrationsofthesubstancestobemeasured.Toaddressthisissue,anadaptiveboundarydetectionpeaksearchalgorithmbasedonwavelettransformisproposed.Thedataisfirstpre-processed,followedbywaveletdecomposition.Bychangingthedecompositionscaleandthenumberoflayers,theinterferencelayercoefficientsaresettozeroandtheeffectivesignalisretained.Theprocessedsignalisthenperformfeatureextraction,andfeatureextractionisachievedbylocalextremevalueidentificationofridges.Finally,thelocalizationofcontrol(C)peakandtest(T)peakisachievedandthepeakareaiscalculated.Theexperimentalresultsshowedthatgoodlinearityintherangeof50to1000pg/mlandthelinearcorrelationcoefficientwasnotlessthan0.998.Therelativestandarddeviationswere1.23%~3.93%.Theresultsshowthattheproposedadaptiveboundarydetectionalgorithmismorecapableofidentifyingweakpeaksandimprovestheaccuracyofpeakmaplocalization.Keywords:Fluorescenceimmunochromatography,Wavelettransform,Borderadaptive,Detectionofpeaks基于自适应边界检测的荧光免疫层析峰图定位研究范昭阳1，刘新全1,2，胡琛光3，张华4，汪曣1，杜康2*（1.天津大学精密仪器与光电子工程学院，天津300072；2.天津博硕科技有限公司，天津300499；3.北京华科泰生物技术有限公司，北京101100；4.天津医科大学总医院空港医院，天津3003081）ResearchonfluorescenceimmunochromatographypeakmaplocalizationbasedonadaptiveboundarydetectionFANZhaoyang1,LIUXinquan1，2,HUChenguang3,ZHANGHua4,WANGYan1,DUKang2*(1.SchoolofPrecisionInstrumentsandOptoelectronics,TianjinUniversity,Tianjin300072;2.TianjinBoshuoTechnologyCo.,Ltd,Tianjin300499;3.BeijingSavantBiotechnologyCo.,Ltd.,Beijing101100;4.TianjinMedicalUniversityGeneralAirportHospital,Tianjin300308)摘要：针对目前荧光免疫层析分析仪对低浓度待测物的T峰寻峰准确率较低的问题，提出了一种基于小波变换的自适应边界检测寻峰算法。首先对数据进行预处理，之后进行小波分解，通过改变分解尺度与层数，将干扰层系数置零且保留有效信号，对处理后的信号进行特征提取。特征提取利用局部极...
