组织培养技术组织培养技术TissueCultureTissueCultureTechniquesTechniques(InVitroCulture)(InVitroCulture)概述概述ⅠⅠ..绪言绪言/Introduction/IntroductionⅡⅡ..细胞生长的根本需要细胞生长的根本需要/Basicrequirementofcell/BasicrequirementofcellgrowthgrowthⅢⅢ..细胞培养的配备细胞培养的配备/Equipment/EquipmentⅣⅣ..清洁、消毒与灭菌清洁、消毒与灭菌/Cleaning,Disinfectionand/Cleaning,DisinfectionandSterilizationSterilizationⅤⅤ..无菌操作无菌操作/Aseptictechnique/AseptictechniqueⅥⅥ..培养细胞的形态培养细胞的形态/Morphologyofculturecell/MorphologyofculturecellI.I.绪言绪言//IntroductionIntroduction1.1.概念概念/Concept/Concept2.2.开展史开展史/Developinghistory/Developinghistory3.3.应用应用/Application/Application1.1.概念概念体外培养体外培养/Invitroculture/Invitroculture————将动植物活体内取出的组织、细胞或器官等，放置将动植物活体内取出的组织、细胞或器官等，放置在类似体内生存环境中，使其维持、生长或繁殖的方在类似体内生存环境中，使其维持、生长或繁殖的方法。法。wholeculturemicroorganismwholeculturemicroorganismparasiteparasiteorgancultureorganculturepartsculturetissueculturepartsculturetissueculturecellculturecellculture器官培养器官培养/Organculture/Organculture体外器官原基或整个器官或局部器官的维持或体外器官原基或整个器官或局部器官的维持或生长，包括结构和功能上的维持和分化。生长，包括结构和功能上的维持和分化。〔〔ThemaintenanceorgrowthoforganThemaintenanceorgrowthoforganprimordiaorthewholeorpartsofanprimordiaorthewholeorpartsofanorganinvitro,inawaythatmayalloworganinvitro,inawaythatmayallowdifferentiationandpreservationofthedifferentiationandpreservationofthearchitectureand/orfunction.architectureand/orfunction.〕〕OrgancultureOrganculture•组织培养组织培养/Tissueculture/Tissueculture体外组织的维持或生长，包括结构和功能上的维体外组织的维持或生长，包括结构和功能上的维持和分化。持和分化。〔〔ThemaintenanceorgrowthofThemaintenanceorgrowthoftissueinvitro,inawaythatmaytissueinvitro,inawaythatmayallowdifferentiationandallowdifferentiationandpreservationofthearchitecture...
