医学分子生物学杂志,2023,20(5):432-438JMedMolBiol,2023,20(5):432-438DOI:10.3870/j.issn.1672-8009.2023.05.009资助项目:南充市科技项目应用基础课题研究(No.19SXHZ0323)通讯作者:王攀(电话:13990768533,E-mail:ncwangpan@126.com)ThisworkwassupportedbyagrantfromtheApplicationofScienceandTechnologyResearchProjectsinNanchong(No.19SXHZ0323)Correspondingauthor:WANGPan(Tel:86-13990768533,E-mail:ncwan-gpan@126.com)癌相关成纤维细胞外泌体miR-34c-5p对胃癌细胞干细胞样表型的影响徐秀连,谢平,印隆宽,袁华燕,刘建军,王攀川北医学院附属医院胃肠外一科四川省南充市,637000【摘要】目的探讨癌相关成纤维细胞(cancer-associatedfibroblasts,CAFs)来源的外泌体miR-34c-5p是否有助于促进胃癌(gastriccarcinoma,GC)细胞的干细胞样特性。方法收集并鉴定初代培养的CAFs和配对的正常成纤维细胞(normolfibroblasts,NFs)的外泌体,检测CAFs和NFs之间外泌体中miR-34c-5p的差异表达。CCK8实验检测GC细胞活力;克隆形成实验检测GC细胞增殖能力;Transwell实验检测GC细胞侵袭能力;细胞成球形成实验评估GC细胞成球能力;蛋白质印迹法检测细胞干细胞特性相关蛋白的表达。结果miR-34c-5p在CAFs衍生的外泌体中的表达显著降低。与CAFs-exo组比较,CAFs-exo中miR-34c-5p抑制GC细胞的增殖和侵袭能力(P<0.05);抑制GC细胞成球,并降低SOX2、OCT4和NANOG蛋白的表达水平,有显著性差异(P<0.05)。结论CAFs来源的外泌体miR-34c-5p抑制GC细胞的干细胞样表型。【关键词】癌相关成纤维细胞;外泌体;胃癌;miR-34c-5p;干细胞特性【中图分类号】R735.2EffectofCancer-associatedFibroblastExosomalmiR-34c-5ponGas-tricCancerStemCell-likePhenotypeXUXiulian,XIEPing,YINLongkuan,YUANHuayan,LIUJianjun,WANGPanDepartmentofGastrointestinalSurgery,AffiliatedHospitalofNorthSichuanMedicalCollege,Nan-chong,637000,China【Abstract】ObjectiveToinvestigatetheeffectofexosomalmiR-34c-5pderivedfromcancer-associatedfibroblasts(CAFs)onthestem-likepropertiesofgastriccancer(GC)cells.MethodsExosomesfromprimaryculturedCAFsandpairednormalfibroblasts(NFs)werecollectedandi-dentified,andthedifferentialexpressionofexosomalmiR-34c-5pbetweenCAFsandNFswasde-tected.TheviabilityofGCcellswasdetectedbyCCK8assay.TheproliferationofGCcellswasde-tectedbycolonyf...
