基金项目：贵州省科技计划项目《遵义市综合检验检测创新公共服务平台能力提升》黔科中引地[2021]4008。DOI：10.13746/j.njkj.2022279HPLC同时测定酱香型白酒酒糟中13种有机酸的分析研究侯睿，向丽萍，张冰雪，任伟，张琴，骆书娜*（遵义市产品质量检验检测院，贵州遵义563099）摘要：有机酸对酱香型白酒的香气、味觉和口感有重要的影响，酱香型白酒显著特征表现为“酸高”。为进一步加强白酒中有机酸的品质控制，该研究建立了高效液相色谱(HPLC)法同时测定白酒酒糟中13种有机酸的方法，实现了多种有机酸之间有效分离。结果表明，该方法测定13种有机酸在0.000158～0.0947mg/mL范围内线性关系良好(R2＞0.99)，峰面积重复性相对标准偏差(RSD)＜5%，加标回收率84.0%～111.0%。采用该方法对同一企业不同车间七轮次酒酒糟进行检测，能准确测定出不同酒糟中13种有机酸的差异，该方法简单快捷、准确可靠，重现性好，证明该方法可用于实际生产中酱香型白酒酒糟中13种有机酸的含量检测。关键词：高效液相色谱法；有机酸含量；白酒酒糟中图分类号：：TS262.3；TS261.7文献标识码：A文章编号：1001-9286（2023）08-0103-05SimultaneousDeterminationof13OrganicAcidsinFermentedGrainsofJiangxiangBaijiubyHPLCHOURui,XIANGLiping,ZHANGBingxue,RENWei,ZHANGQinandLUOShuna*(ZunyiProductQualityInspectionandTestingInstitute,Zunyi,Guizhou563099,China)Abstract:Organicacidshaveimportantinfluenceonthearoma,tasteandflavorofJiangxiangBaijiu,whichischaracterizedbyhighacidity.InordertocontroltheorganicacidcontentinBaijiu,anHPLCmethodwasestablishedforsimultaneousdeterminationof13organicacidsinthefermentedgrainsofBaijiu,andeffectiveseparationofmultipleorganicacidswasrealized.Thelinearityofthemethodwasgoodintherangeof0.000158mg/mLto0.0947mg/mL(R2>0.99),therelativestandarddeviation(RSD)waslessthan5%,andthespikedrecoverieswereintherangeof84.0%to111.0%.Themethodwasusedtotestthefermentedgrainsfromdiffer-entworkshopsofthesamedistillery,andtheirdifferencesinorganicacidscouldbeaccuratelydetermined.Themethodissimple,fast,accurateandreliable,andhasgoodreproducibility,whichcanbeusedforthedetectionofthe13organicacidsinthefermentedgrainsofJiangxiangBaijiuinactualproduction.Keywords:highperformancel...
