RoleandmechanismofPDGFRAinregulatingp53signalingpathwaytomediateproliferation，apoptosisandinvasionofovariancancercellsXIONGTingchuan1，WANGYinghong2，ZHUChangjun3（1.DepartmentofGynecologicSurgery，The3rdAffiliatedTeachingHospitalofXinjiangMedicalUniversity（AffiliatedCancerHospital），Urumqi830011，China；2.CenterofHeathManagement，TheFirstAffiliatedHospitalofXinjiangMedicalUniversity，Urumqi830011，China；3.TianjinKeyLaboratoryofAnimalandPlantResistance，TianjinNormalUniversity，Tianjin300387，China）Abstract：Toinvestigatetheroleandspecificmechanismofplateletderivedgrowthfactorreceptorα（PDGFRA）inregulat-ingtheproliferation，apoptosisandinvasionofovariancancercells，differentialexpressionofPDGFRAinovariancancerwasverifiedfirstlybasedontheanalysisofrelevantgenechips（GSE4122andGSE14407）intheGEOdatabase.ThenSangerBoxwasusedtoanalysetheassociationbetweenPDGFRAandtheprognosisofovariancancerpatientsonline.ThePDGFRAover-expressionplasmidwascreated，theeffectofPDGFRAonOVCAR3cellproliferationwasexploredusingMTT，theeffectofPDGFRAonOVCAR3cellapoptosiswasdetectedusingflowcytometry，theeffectofPDGFRAonOVCAR3cellinvasionwasdetectedusingTranswell，andtheexpressionlevelsofp-p53，p53andp21proteinsweremeasuredusingWesternblot.TheresultsshowedthatPDGFRAexpressionwassignificantlyup-regulatedinovariancancer，andpatientswithhighPDGFRAexpressionhadaworseprognosiscomparedtothosewithlowPDGFRAexpression.Cellproliferationandinvasionweresignifi-cantlyhigherinthePAGFRAgroupcomparedtovector-NCgroup，whileapoptosisandp-p53andp21proteinexpressionlevelsweresignificantlylower.ItisconcludedthatPDGFRAcaninfluenceOVCAR3cellproliferation，apoptosisandinvasionbyme-diatingtheregulationofthep53signallingpathway.PDGFRA调控p53信号通路介导卵巢癌细胞增殖、凋亡和侵袭的作用及机制熊廷川1，王迎洪2，朱长军3（1.新疆医科大学第三临床医学院（附属肿瘤医院），妇外三科，乌鲁木齐830011；2.新疆医科大学第一附属医院健康管理中心，乌鲁木齐830011；3.天津师范大学天津市动植物抗性重点实验室，天津300387）摘要：为了探究血...
