·976·第53卷第8期2023年8月Vol.53No.8Aug.2023日用化学工业（中英文）ChinaSurfactantDetergent&CosmeticsReceived:August3,2022;Revised:July28,2023.*Correspondingauthor.E-mail:xuweichang@sinochem.com（WeichangXu）;wangyuan@sinochem.com（YuanWang）.DOI：10.3969/j.issn.2097-2806.2023.08.015DeterminationofasiaticosideandmadecassosideinCentellaasiatica（L.）UrbanbyHPLC-ELSDHongdanWang1,WeichangXu2,*,YuanWang2,*,XiangwenKong1,LijuanSun2（1.CollegeofScience,ShenyangUniversityofChemicalTechnology,Shenyang,Liaoning110000,China;2.ShenyangChemicalResearchInstituteCo.,Ltd.,Shenyang,Liaoning110000,China）Abstract:Centellaasiatica（L.）Urbanextractisusuallyusedasacosmeticrawmaterial,whichhaswhitening,antioxidantandothereffects.Themainsubstancestoplaythiseffectareasiaticosideandmadecassoside.Inthispaper,ahighperformanceliquidchromatography-evaporativelightscatteringdetection（HPLC-ELSD）techniquewasusedtodeterminethecontentofasiaticosideandmadecassosideinCentellaasiatica（L.）Urban.UnitaryC18column（4.6×250mm,5μm）withacetonitrile-waterasthemobilephasewasusedforgradientelutionataflowrateof1mL/min.Columntemperaturewas30℃.Agilent1260InfinityⅡdetector（drifttubetemperaturewas70℃）wasusedforcolumnelutionwiththecarriergasflowrateat1.6L/min.Theresultsshowthattheconcentrationsofasiaticosideandmadecassosideare100-702mg/Land105-738mg/L,respectively.Thelinearrelationshipbetweenpeakareaandconcentrationisgood,andthecorrelationcoefficientsare0.9996and0.9997,respectively.Theaveragerecoveriesofasiaticosideandmadecassosideare101.20%and98.30%,respectively.Therelativestandarddeviations（RSD）areallwithin3%.Themethodissimple,accurate,effectiveandlessinterference,andcanbeusedforthedeterminationofasiaticosideandmadecassosideinCentellaasiatica（L.）Urban.Keywords:Centellaasiatica（L.）Urban;highperformanceliquidchromatography-evaporativelightscatteringdetector;asiaticoside;madecassoside;contentdetermination05101520253035140A101530202535405045051015202530351340C50010015025020020510152025303540B02004...
