∗doi:10.3969/j.issn.2095-1736.2023.04.098Dof转录因子在作物逆境胁迫响应及农艺性状改良中的作用王泽民1,晋昕1,张飞燕1,司怀军1(甘肃农业大学生命科学技术学院,兰州730070)摘要阐述DNA-bindingwithonefinger(Dof)转录因子在作物中应答极端温度、干旱和盐胁迫等逆境的分子机制及其改良农艺性状的作用;介绍Dof转录因子在作物中响应逆境的信号感知方式和信号转导途径,分析其基因表达调控过程的调控机理以及对胁迫耐受力与重要次生代谢产物(RFOs等)等积累的影响。同时从多信号网络调控机制以及全球气候变化对作物可持续和高质量生产的需求角度,对进一步研究和利用Dof转录因子进行作物生物育种的前景和方向作展望。关键词生物育种;Dof转录因子;转录调控;非生物胁迫;农艺性状中图分类号S763文献标识码A文章编号2095-1736(2023)04-0098-09TheroleofDoftranscriptionfactorsincropstressresponseandagronomictraitimprovementWANGZemin1JINXin1ZHANGFeiyan1SIHuaijun1CollegeofLifeScienceandTechnologyGansuAgriculturalUniversityLanzhou730070ChinaAbstractThemolecularmechanismsofDNA-bindingwithonefingerDoftranscriptionfactorsincropresponsetostresssuchasex-tremetemperaturedroughtandsaltstressandtheirimportantrolesinagronomictraitimprovementwereanalyzed.CombinedwiththeeffectsofdifferentabioticstressesonplantgrowthanddevelopmentthesignalsensingmodeandsignaltransductionpathwayofDoftranscriptionfactorsincropresponsetostresswereintroduced.TheregulationmechanismofDoftranscriptionfactorsandtheactionmodeofstresstoleranceandimportantsecondarymetabolitesRFOsetc.duringtheregulationofgeneexpressionwereanalyzed.Moreoverfromtheperspectiveofmulti-signalnetworkregulationmechanismandthedemandofglobalclimatechangeforsustainableandhigh-qualitycropproductiontheprospectsanddirectionsoffurtherresearchandutilizationofDoftranscriptionfactorsincropbio-breedingwereprospected.Keywordsbio-breedingDoftranscriptionfactorstranscriptionalregulationabioticstressagronomictrait转录因子(transcriptionfactors,TF)是一种蛋白质,它结合在目标基因启动子区域的特定DNA基序上,调节其转录。植物体内近7%的基因转录产生TFs,已知TFs参与应对干旱、渗透、高温和低温等非生物胁迫[1-3]。植物TFs根据其DNA结合...
