安徽农学通报2023年02期病原菌·有害生物·动植物防护烟草黑胫病生防功能菌筛选及其应用效果郝浩浩1李翔1郭传滨1吴俊林1唐培培1赵佳佳1孙会忠2（1河南省烟草公司驻马店市公司，河南驻马店463000；2河南科技大学农学院，河南洛阳471000）摘要以野生络石Trachelospermumjasminoides为试验材料，采用组织平板法从其植株体内分离培养出31株内生细菌活体纯培养物，以烟草黑胫病原Phytophthoraparasiticavar.nicotianae为指示菌株筛选出的HHH12具有明显的抑菌作用，抑菌圈直径达到24mm，初步定性为具有生防活性菌株。HHH12的多相鉴定结果为假单孢菌属Pseudomonas菌株，暂定名Pseudomonassp.HHH12。盆栽试验证实，HHH12菌悬液的灌根+叶面喷雾混合处理对烟草黑胫病不同发病阶段均表现出比叶面喷雾和灌根单一处理有更好的应用效果，药后14d时的防治效果达到72.86%。菌株HHH12具有良好的开发应用前景。关键词烟草黑胫病；生防菌；抑菌活性中图分类号S47文献标识码A文章编号1007-7731（2023）02-0105-05ScreeningandApplicationofBiocontrolBacteriaforTobaccoBlackShankDiseaseHAOHaohaoLIXiangGUOChuanbinWUJunlinTANGPeipeiZHAOJiajiaSUNHuizhong（1ZhumadianBranchofHenanProvincialTobaccoCompany,ZhumadianHenan463000;2AgriculturalCollegeofHenanUniversityofScienceandTechnology,LuoyangHenan471000）AbstractUsingTrachelospermumjasminoidesasmaterial,31strainsofendophyticbacteriawereisolatedandculturedinvivobytissueplatemethod.ThestrainHHH12wasscreenedoutwithPhytophthoraparasiticavar.nicotianaeastheindicator.Thediameteroftheinhibitionzoneswas24mm,itwaspreliminarilyidentifiedasastrainwithbiocontrolactivity.PolyphasicidentificationofHHH12wasPseudomonassp.HHH12.PotexperimentsconfirmedthatHHH12suspensionshowedbetterefficacyintreatingT.jasminoides‘Flame’leafdiseaseatdifferentstagesthanleafsprayandrootsprayalone,andthecontroleffectreached72.86%at14days.StrainHHH12hasagooddevelopmentandapplicationprospect.KeywordsPhytophthoraparasiticavar.nicotianae;biocontrolbacteria;antibacterialactivity络石（T.jasminoides）属夹竹桃科络石属，是一种传统中草药植物，有抑菌、抗痛风之效，临床多用于治疗坐骨神经、关节炎、喉痹咽塞、喘息不通、咳嗽喘息、腹泻等症[1]。除具有药理作用外，络石也是常见...
