·39·国际消化病杂志2023年2月第43卷第1期IntJDigDis，February25，2023，Vol.43，No.1·论著·健脾通络方对结直肠癌细胞侵袭迁移的影响及机制研究黄人可任建琳靖琳吴杏黎刘湘君刘怡储金砚【摘要】目的探究健脾通络方对结直肠癌细胞侵袭、迁移的影响及机制。方法构建敲减MALAT1基因的HCT116细胞模型。采用细胞划痕实验和Transwell实验检测细胞侵袭、迁移能力。采用蛋白质印迹法检测经健脾通络方干预后的MALAT1正常/敲减表达的HCT116细胞中Wnt3a、β-catenin蛋白水平。采用实时荧光定量PCR法检测MALAT1的表达水平。构建裸鼠肺转移模型，采用小动物活体成像技术监测肿瘤转移情况，并检测转移灶中Wnt3a、β-catenin和MALAT1的表达水平。结果经健脾通络方干预后，HCT116细胞中MALAT1、Wnt3a和β-catenin的表达水平均降低，HCT116细胞迁移能力下降。在裸鼠肺转移模型中，经健脾通络方干预后裸鼠的转移灶减少，转移灶中Wnt3a、β-catenin和MALAT1的表达水平均降低。结论健脾通络方通过下调HCT116细胞中MALAT1的表达，使Wnt/β-catenin信号通路中Wnt3a、β-catenin的蛋白表达水平降低，从而抑制结直肠癌细胞的增殖、侵袭和迁移能力。【关键词】结直肠癌；侵袭；迁移；MALAT1；Wnt/β-catenin信号通路DOI:10.3969/j.issn.1673-534X.2022.06.009EffectofJianpiTongluoDecoctiononinvasionandmigrationofcolorectalcancercellsanditsmechanismHUANGRenke,RENJianlin,JINGLin,WUXingli,LIUXiangjun,LIUYi,CHUJinyan.DepartmentofOncology,ShanghaiMunicipalHospitalofTraditionalChineseMedicine,ShanghaiUniversityofTraditionalChineseMedicine,Shanghai200071,China【Abstract】ObjectiveAnattemptismadeinthispapertoinvestigatetheeffectandmechanismofJianpiTongluoDecoctiononinvasionandmigrationofcolorectalcancercells.MethodsTheMALAT1knockdownHCT116cellmodelwasconstructed.CellscratchtestandTranswelltestwereconductedtodetectcellinvasionandmigration.WesternblottingwasconductedtodetectWnt3aandβ-cateninproteinlevelsinHCT116cellswithnormal/knockdownexpressionofMALAT1aftertreatmentwithJianpiTongluoDecoction.TheexpressionofMALAT1wasdetectedbyreal-timefluorescencequantitativePCR.Thelungmetastasismodelofnudemicewasestablished,andthetumormetastasiswasmonitoredbyutilizingthesmallanimalinvivoimagingtechnology.T...
