ICS65.020B16DB35福建省地方标准DB35/T1280—2012大豆疫霉菌分子检测技术规程StandardprotocolformoleculardetectionofPhytophthorasojae2012-11-02发布2013-02-01实施福建省质量技术监督局发布DB35/T1280—2012I目次前言...............................................................................III1范围..............................................................................12规范性引用文件....................................................................13术语和定义........................................................................14原理..............................................................................15材料与试剂........................................................................15.1主要仪器与器材................................................................15.1.1PCR检测仪.................................................................15.1.2凝胶成像仪................................................................25.1.3台式离心机................................................................25.1.4涡旋混匀器................................................................25.1.5电泳仪....................................................................25.2主要试剂......................................................................25.2.1苯酚；氯仿；异戊醇；乙醇..................................................25.2.2二甲基亚砜................................................................25.2.3氢氧化钠..................................................................25.2.4脱脂奶粉..................................................................25.2.5PCR常规试剂...............................................................26采样..............................................................................26.1采样工具......................................................................26.2样品采集......................................................................26.2.1大豆植株....................................................................
