ICS11.220CCSB41DB51四川省地方标准DB51/T2906—2022猪盖塔病毒分离与鉴定技术规范Technicalspecificationforisolationandidentificationofswinegaetavirus2022-05-20发布2022-07-01实施四川省市场监督管理局发布DB51/T2906—2022I目次前言.................................................................................II1范围...............................................................................12规范性引用文件.....................................................................13术语和定义.........................................................................14缩略语.............................................................................15试剂与设备.........................................................................1主要试剂.......................................................................15.1主要仪器设备...................................................................25.26样品的采集、保存与运输.............................................................2猪只临床发病特征...............................................................26.1临床样品的采集.................................................................26.2保存与运输.....................................................................26.37病毒的细胞分离培养.................................................................2生物安全措施...................................................................27.1样品的处理.....................................................................27.2单层细胞制备...................................................................27.3接种细胞.......................................................................27.4病毒收获.......................................................................37.58病毒核酸RT-PCR鉴定................................................................3核酸提取和反转录...............................................................38.1RT-PCR扩增.....................................................................38.29结果判定...........
