《食品工业》2023年第44卷第4期122工艺技术青楷槭茎皮乙醇提取工艺优化和抗氧化性研究熊思瑞,金铁岩*延边大学农学院(延吉133002)摘要为优化青楷槭茎皮乙醇提取的工艺条件并研究其抗氧化能力、进一步分离青楷槭茎皮生物活性成分、丰富槭树科的利用价值,利用正交试验对青楷槭茎皮乙醇提取条件进行优化,并通过测定最优条件提取物的DPPH·、ABTS+·两种自由基的清除率以评价青楷槭茎皮醇提物的抗氧化作用。结果表明,青楷槭茎皮乙醇提取物最优提取条件为料液比1∶10(g/mL)、乙醇体积分数60%、浸泡时间6h,在最优条件下青楷槭茎皮醇提物的总酚含量为577.8μg/mg,并且当青楷槭茎皮醇提物浓度达到500μg/mL时,DPPH·清除率为89.26%,ABTS+·清除率为92.98%。通过与BHA对比发现,其抗氧化作用随浓度升高而增强,呈现出剂量效应关系,表明青楷槭茎皮醇提物具有良好抗氧化能力。关键词青楷槭;工艺优化;抗氧化作用StudyonEthanolExtractionProcessOptimizationandAntioxidantActivityofStemPeelofAcertegmeutosumMaximXIONGSirui,JINTieyan*CollegeofAgriculture,YanbianUniversity(Yanji133002)AbstractInordertooptimizetheextractionprocessofethanolfromstempeelofAcercymbiumandstudyitsantioxidantcapacity,tofurtherisolatethebiologicalactivecomponentsfromthestembarkofAcercydendrum,andtoenrichtheutilizationvalueofAceraceae,orthogonalexperimentwasusedtooptimizetheethanolextractionconditionsofAcercydendrumstempeel,andtheantioxidantactivityoftheethanolextractfromAcercydendrumstempeelwasevaluatedbymeasuringthescavengingratesofDPPH·andABTS+·oftheoptimalextract.Theresultsshowedthattheoptimalextractionconditionsweresolid-liquidratioof1∶10(g/mL),ethanolconcentrationof60%andsoakingtimeof6h.Undertheoptimalconditions,thetotalphenoliccontentoftheethanolextractwas577.8μg/mg.Whentheethanolconcentrationreached500μg/mL,theDPPH·scavengingratewas89.26%,andtheABTS+·scavengingratewas92.98%.ComparedwithBHA,theantioxidantactivityincreasedwithincreasingconcentration,showingadose-effectrelationship,indicatingthattheethanolextractsfromstembarkofAcercydendrumhadgoodantioxidantcapacity.KeywordsAcertegmeutosumMaxim;processoptimization;antioxidanteffect*通信作者青楷槭(AcertegmeutosumMa...