草地贪夜蛾体表靶向TRPV1的毒素分子筛选及其功能研究刘嘉逸1,张浩源1,冯雯灵1,贾佩君1,加依达古丽·格明1,尹传玲1,柴龙会1,陆先翠1,王云飞1,马志朋2∗(1.东北林业大学野生动物与自然保护地学院,黑龙江哈尔滨150040;2.唐山动物园,河北唐山063021)摘要[目的]探究草地贪夜蛾诱发人畜危害的分子机制,以草地贪夜蛾诱发人畜呼吸道炎症、疼痛等危害的潜在生理靶点(瞬时电位感受器香草酸受体1,TRPV1)为切入点,揭示草地贪夜蛾体表物质诱发人畜危害的毒素物质基础和生理靶点。[方法]通过凝胶分子筛以及高效液相色谱等方法对草地贪夜蛾体表物质进行分离和纯化,结合膜片钳电生理技术筛选草地贪夜蛾体表物质中靶向TRPV1的活性毒素组分。[结果]从草地贪夜蛾体表物质中分离纯化到一种能够高效(100mg/L)激活TRPV1的毒素组分V-7,该组分能特异性作用于TRPV1,而对常见离子通道类膜蛋白生理受体(如钠离子通道NaV1.4、NaV1.5、NaV1.7,以及钾离子通道KV1.1、KV2.1、KV4.1)没有作用。[结论]从草地贪夜蛾体表混合物中可分离得到一种高效且特异性激活动物生理靶点TRPV1的毒素组分,部分揭示了外来入侵农业害虫草地贪夜蛾诱发人畜危害的物质基础及生理靶点。关键词草地贪夜蛾;体表物质;TRPV1;毒素中图分类号S433.4文献标识码A文章编号0517-6611(2023)11-0065-05doi:10.3969/j.issn.0517-6611.2023.11.015开放科学(资源服务)标识码(OSID):MolecularScreeningandFunctionalInvestigationofToxinsTargetedtoTRPV1intheBodySurfaceofSpodopterafrugiperdaLIUJia-yi,ZHANGHao-yuan,FENGWen-lingetal(CollegeofWildlifeandNatureReserves,NortheastForestryUniversity,Harbin,Heilongjiang150040)Abstract[Objective]Inordertoexplorethemolecularmechanismofhuman-animalhazardsinducedbythefallarmyworm,thepotentialphysiologicaltargets(transientreceptorpotentialvanillicacidsubtype1,TRPV1)inducinghumanandanimalrespiratoryinflammationandpainwereusedastheentrypoint,andthetoxinmaterialbasisandphysiologicaltargetsofthebodysurfacesubstanceofSpodopterafrugiperdawereusedastheentrypointtorevealthetoxinsubstancebasisandphysiologicaltargetofthehumanandanimalhazardsinducedbythemateri-alonthesurfaceofSpodopterafrugiperda.[Method]Thebodysurfacesubstanceofthegrassmothwasisolatedandpurifiedbygelmolecularsieveandhighperformanceliquidchromatography...
