韩鹏飞,朱璇,杨敏,等.戴氏虫草产多糖的固体发酵工艺优化[J].食品工业科技,2023,44(14):130−136.doi:10.13386/j.issn1002-0306.2022090117HANPengfei,ZHUXuan,YANGMin,etal.Solid-stateFermentationofCordycepstaiiforPolysaccharideProduction[J].ScienceandTechnologyofFoodIndustry,2023,44(14):130−136.(inChinesewithEnglishabstract).doi:10.13386/j.issn1002-0306.2022090117·生物工程·戴氏虫草产多糖的固体发酵工艺优化韩鹏飞1,2,朱璇1,杨敏1,黄贵强1,*(1.六盘水师范学院生物科学与技术学院,贵州六盘水553000;2.大连大学生命健康学院,辽宁大连116622)摘要:以大米为原料进行戴氏虫草真菌发酵,通过优化发酵条件提高戴氏虫草多糖含量。采用单因素实验和多因素实验优化戴氏虫草产多糖的固体发酵条件。Plackett-Burman试验结果表明,影响戴氏虫草多糖含量的主要因素是发酵温度、接种量和料水比。最陡爬坡试验和Box-Behnken试验得出产戴氏虫草多糖的最适条件为:发酵温度25℃,接种量8%,料水比(g/mL)=1:1.5,基质重量10g,发酵时间5d,得到的戴氏虫草多糖含量为5.31%±0.11%,与理论值的误差为0.93%±1.8%。本文旨在为戴氏虫草多糖的工业化生产提供理论依据,同时为其它虫草真菌固体发酵生产虫草多糖提供理论参考。关键词:戴氏虫草,固体发酵,大米,多糖,条件优化本文网刊:中图分类号:Q946.92文献标识码:A文章编号:1002−0306(2023)14−0130−07DOI:10.13386/j.issn1002-0306.2022090117Solid-stateFermentationofCordycepstaiiforPolysaccharideProductionHANPengfei1,2,ZHUXuan1,YANGMin1,HUANGGuiqiang1,*(1.CollegeofBiologicalScienceandTechnology,LiupanshuiNormalUniversity,Liupanshui553000,China;2.CollegeofLifeandHealth,DalianUniversity,Dalian116622,China)Abstract:RicewasusedasmaterialsforCordycepstaiifermentation,thecontentofCordycepstaiipolysaccharidewasincreasedthroughoptimizingfermentationconditions.Single-factorandmulti-factorexperimentswereusedtooptimizethesolidfermentationconditionsofCordycepstaiiproducingpolysaccharide.TheresultsofPlackett-Burmantestshowedthatfermentationtemperature,inoculationquantityandtheratioofmaterialtowaterweremainaffectingfactorsforthecontentofCordycepstaiipolysaccharide.ThenthesteepestascentexperimentandBox...