第36卷第4期,2023年7月宁波大学学报（理工版）中国科技核心期刊Vol.36No.4,July2023JOURNALOFNINGBOUNIVERSITY(NSEE)中国高校优秀科技期刊DOI:10.20098/j.cnki.1001-5132.2023.0414UricacidmediatedhypomethylationofOPRD1geneaffectsAlzheimer’sdiseaseCHENYao1,2,CHENYong1*,YINGYing1,FANJianbo3,GENGBaoqing1,ZHENGChengying4,XUTing4,FUHaoyuan5,ZHANGYunge6,CHENJi6(1.NingboNo.2Hospital,Ningbo315010,China;2.TaizhouHospitalofZhejiang,Taizhou317099,China;3.ZhenhaiLongsaiHospital,Ningbo315299,China;4.NingboKangningHospital,Ningbo315201,China;5.TheThirdPeople’sHospitalHealthCareGroupofCixi(ChangHeBranch),Ningbo315325,China;6.HealthScienceCenter,NingboUniversity,Ningbo315211,China)Abstract:ThepresentstudywasaimedtoinvestigatethecorrelationbetweenOPRD1genemethylation,uricacidandAlzheimer’sdisease.ItalsoexaminedwhetheruricacidcouldaffectOPRD1genemethylationinvitro.ThevenousbloodofthepatientswithAlzheimer’sdisease,thehyperuricemiagroupandthehealthycontrolgroupwascollected.ThecellsweresuccessivelytreatedwithdifferentconcentrationsofuricacidandthemethylationlevelofOPRD1genewassubsequentlydetermined.ThemethylationlevelsofOPRD1atpos2andpos4inthehyperuricemiagroupwerelowerthanthoseofthehealthycontrolgroup;whilethemethylationlevelofpos4intheAlzheimer’sdiseasegroupwashigherthanthatofthehealthycontrolgroup.UricacidlevelswerenegativelycorrelatedwithOPRD1genemethylationlevels.Invitro,themethylationlevelsatpos2,pos3andpos4sitesofOPRD1geneincellswithhighconcentrationofuricacidwerelowerevenfurther.ThepresentstudysuggestedthaturicacidmayaffecttheonsetofAlzheimer’sdiseasebymediatingthehypomethylationmodificationatthepos4siteofOPRD1gene.Keywords:uricacid;keyenzymes;OPRD1gene;methylation;Alzheimer’sdiseaseCLCnumber:R741Documentcode:AArticleID:1001-5132（2023）04-0078-111IntroductionAlzheimer’sdisease(AD),themostcommonneurodegenerativediseaseleadingtoseniledementia,isamajorpublichealthproblemworthyoftheattentionofthewholesociety.RecentstudieshavefoundthathypermethylationmodificationoftheOPRD1geneisassoc...
