分子植物育种，2023年，第21卷，第14期，第4679-4684页MolecularPlantBreeding,2023,Vol.21,No.14,4679-4684研究报告ResearchReport基于高密度遗传连锁图谱的稻谷粒重QTL定位张大双1彭强1*李佳丽1季家举2徐海峰1朱速松11贵州省农业科学院贵州省水稻研究所,贵阳,550006;2湖南轻工研究院有限责任公司,长沙,410015*通信作者,pengqiang_as@163.com摘要为挖掘稳定遗传的稻谷粒重性状QTL，本研究以V20B/CPSLO17组合衍生的150个重组自交家系(recombinantinbredline,RIL)为作图群体，在3个环境(2019贵阳,2020贵阳,2019三亚)对稻谷粒重性状进行QTL检测及其遗传效应分析。结果表明：3个环境共检测到6个稻谷粒重QTL，其中QTLqTGW5-1在2种环境被重复检测到；QTLqTGW5-2和qTGW5-3具有较大遗传效应，表型变异贡献率高达139.796%和99.414%，两者的LOD值分别为35.113和28.411。qTGW5-2的加性效应源自亲本CPSLO17；qTGW5-3的加性效应源自亲本V20B。本研究结果为挖掘新的稻谷粒重性状基因提供参考依据。关键词水稻(OryzasativaL.);环境;粒重;作图群体;基因QTLsMappingofRiceGrainWeightBasedonHigh-densityGeneticMapZhangDashuang1PengQiang1*LiJiali1JiJiaju2XuHaifeng1ZhuSusong11GuizhouRiceResearchInstitute,GuizhouAcademyofAgriculturalSciences,Guiyang,550006;2HunanLightIndustryInstitute,Changsha,410015*Correspondingauthor,pengqiang_as@163.comDOI:10.13271/j.mpb.021.004679AbstractInordertoexplorethestablegeneticQTLsforgrainweighttraitsofrice,150recombinationinbredlines(RILs)derivedfromV20B/CPSLO17combination,wereappliedtodetectQTLsforgrainweighttraitsandanalyzetheirgeneticeffectsinthreeenvironments(2019Guiyang,2020Guiyang,2019Sanya).TheresultsshowedthatatotalofsixQTLsforgrainweighttraitsweredetestedinthreeenvironments,ofwhichtheQTLqTGW5-1wasrepeatedlydetectedintwoenvironments.QTLsqTGW5-2andqTGW5-3hadmajorgeneticeffects,andthecontributionrateofphenotypicvariationwasashighas139.796%and99.414%,andtheLODvaluesofthetwowere35.113and28.411,respectively.TheadditiveeffectofqTGW5-2wasderivedfromtheparentCPSLO17,andqTGW5-3wasderivedfromtheparentV20B.Theseresultsprovidereferencebasisforfurtherexploitingnewcandidategeneofgrainweightinrice.KeywordsOryzasativaL.;Environment;Grainweigh...
