4期ArylHydrocarbonReceptor-mediatedCardiacToxicityinAdultZebrafishInducedbyTetrabromobisphenolASHANJing1，2，MAXiaofa1，2，WUMengyu1，2，LINYujia1，2，WANGYi1，2，WANGRui1，2，XUHaiming1，2（1.SchoolofPublicHealthandManagement，NingxiaMedicalUniversity，Yinchuan750004，China；2.TheKeyLaboratoryofEnvironmentalFactorsandChronicDiseaseControlofNingxia，Yinchuan750004，China）Abstract：ObjectiveInthisstudy，zebrafish（Daniorerio），amodelorganism，wasusedasanexperimentalanimal，andCH223191（CH），anaromatichydrocarbonreceptor（AhR）inhibitor，wasusedtoexploretheroleofAhRinthecardiactoxiceffectoftetrabromobisphenolA（TBBPA）onzebrafish.MethodsAdultzebrafishwererandomlydividedintosixgroupsforexposure，namelysolventcontrolgroup（DMSO），CHgroup（0.05μmol·L-1），TBBPAexposuregroups（10，100，1000nmol·L-1），andCH+TBBPA（1000nmol·L-1）group.After30daysofexposure，thebodyweightandbodylengthweremeasured.TheexpressionofAhR-relatedgenes（ahr2，cyp1a1andcyp1a2），cardiacfunction-relatedgenes（sox9b，nkx2.5，gate4，myh6，tbx5andvmhc）andreactiveoxygenspecies-relatedgenes（nrf2，gpx1a，sod1，sod2andcat）incardiactissueweredetectedbyqRT-PCR.Theantioxidantenzymes（SOD，CATandGSH-Px/GPX）activitiesinlivertissueweretestedbycommercialkits.ResultsComparedwiththeDMSOcontrolgroup，therewasnosignificantdifferenceinbodyweightandbodylengthinallexposuregroups（Pall>0.05）.TheresultsofqRT-PCRshowedthatAhR-relatedgenes（ahr2，cyp1a1andcyp1a2）wasdown-regulatedintheCHgroup，whileup-regulatedin10，100，1000nmol·L-1TBBPAgroups（Pall<0.05）.Comparedwiththe1000nmol·L-1TBBPAexposuregroup，theseAhRrelatedgenesweredecreasedtoacertainextentintheCH+1000nmol·L-1TBBPAgroup（Pall<0.05）.Thecardiacfunction-relatedgenes（sox9b，nkx2.5，gate4，myh6，tbx5andvmhc）weredown-regulatedinthe1000nmol·L-1TBBPAgroup（Pall<0.05）.Thereactiveoxygenspecies-relatedgenes（gpx1a，sod1，sod2andcat）weredown-regulatedwhilenrf2wasup-regulatedinthe1000nmol·L-1TBBPAgroup（Pall<0.05）.Theresultsoftheantioxidantenzymeact...
