鸡的大小黄白卵泡分离及总RNA的提取目录中文摘要：.................................................................................2关键词：.....................................................................................2Abstract：..................................................................................2前言.............................................................................................21.材料和方法.............................................................................41.1实验动物及其组织.........................................................41.2主要仪器和试剂.............................................................41.2.1实验仪器................................................................41.2.2实验试剂................................................................41.2.3溶液配制................................................................51.3实验操作步骤.................................................................51.3.1取样........................................................................51.3.2大小黄白卵泡的提取............................................61.3.3总RNA的提取........................................................61.3.4总RNA完整性鉴定................................................61.3.5总RNA内基因组DNA的去除................................61.3.6反转录反应............................................................71.3.7PCR反应................................................................711.3.8电泳检测...............................................................71.3.9PCR产物纯化........................................................71.3.10连接转化.............................................................82.实验结论............................................................................82.1鸡卵泡颗粒细胞............................................................82.2总RNA的检测结果........................................................92.3PCR检测...........................................
