第32卷，第3期2023年3月化学分析计量CHEMICALANALYSISANDMETERAGEVol.32,No.3Mar.2023高效液相色谱-荧光检测法测定化妆品中α-三联噻吩陈桂琴，张小媚（广东省药品检验所国家药监局化妆品风险评估重点实验室，广州510663）摘要采用高效液相色谱-荧光检测法测定化妆品中α-三联噻吩。样品经甲醇超声提取20min，以C18色谱柱进行分离，以乙腈-水（体积比为85∶15）为流动相进行洗脱，用荧光检测器测定，色谱峰面积外标法定量。α-三联噻吩的质量浓度在5～200ng/mL范围内与色谱峰面积具有良好的线性关系，相关系数（r）为0.9999，方法的检出限为10ng/g。以霜类、乳液类、水剂类及液态油基类4种空白基质进行加标回收试验，在3种不同加标水平下，回收率为94.6%～98.6%，测定结果的相对标准偏差为0.6%～2.2%（n=6）。该方法样品处理简便、快捷，分析灵敏度高，可用于化妆品中α-三联噻吩的检测。关键词高效液相色谱法；荧光检测器；化妆品；α-三联噻吩中图分类号：O657.7文献标识码：A文章编号：1008-6145(2023)03-0034-05Determinationofα-terthiopheneincosmeticsbyHPLCwithfluorescencedetectorCHENGuiqin,ZHANGXiaomei(GuangdongInstituteforDrugControl,NMPAKeyLaboratoryforSafetyRiskAssessmentofCosmetics,Guangzhou510663,China)Abstractα-Terthiopheneincosmeticswasdeterminedbyhighperformanceliquidchromatography(HPLC)withfluorescencedetector.Thesampleswereextractedwithmethanolfor20min,separatedwithC18chromatographycolumn,elutedwithacetonitrilewater(85∶15)asmobilephase,determinedwithfluorescencedetector,andquantifiedwithexternalstandardmethodofchromatographicpeakarea.Themassconcentrationofα-terthienglhadagoodlinearitywiththechromatographicpeakareaintherangeof5-200ng/mL,thecorrelationcoefficientwas0.9999,andthedetectionlimitofthemethodwas10ng/g.Fourblanksamplesoffrost,lotion,wateragentandliquidoilwereusedasthematrixfortherecoverytest.Underthreedifferentadditionlevels,therecoverieswere94.6%-98.6%,andtherelativestandarddeviationsofdeterminationresultswere0.6%-2.2%(n=6).Theresultsshowthatthemethodissimple,rapid,sensitive,whichcanbeusedforthedeterminationofα-terthiopheneincosmetics.KeywordsHPLC;fluorescencedetector;cosmetics;α-terthiopheneα-三联噻吩(又称为...
