安徽农学通报2023年03期动植物·微生物·食用菌·中药材高温对‘万圣节’花青素代谢的影响薛雅施竹玥李舒敏王婷陈淑婷赵荣*李素华张丽华（宿迁学院，江苏宿迁223800）摘要为研究高温胁迫对多肉植物‘万圣节’花青素代谢的影响，以‘万圣节’扦插苗为材料，对其进行37℃处理，分别处理0、6、12、24、48h，测定各处理下‘万圣节’叶片花青素含量、过氧化物酶活性、多酚氧化酶活性、过氧化物酶基因和多酚氧化酶基因的表达水平。结果表明，在高温胁迫处理的48h内，‘万圣节’叶片花青素含量总体呈现下降的趋势，但前12h内花青素含量无显著变化；‘万圣节’叶片多酚氧化酶活性和过氧化物酶总体均呈现先上升后下降的趋势，且均在24h时达到顶峰；过氧化物酶基因cluster-7148.177和cluster-7148.11277表达水平的变化趋势总体上与过氧化物酶活性的变化较为一致，但是过氧化物酶基因cluster-7148.32645和cluster-7148.1529的变化趋势与过氧化物酶活性的变化趋势不一致。由此可见，高温胁迫处理影响‘万圣节’叶片中花青素的积累、多酚氧化酶和过氧化物酶活性；多酚氧化酶基因cluster-7148.5318、过氧化物酶基因cluster-7148.177和cluster-7148.11277可能在高温引起的‘万圣节’花青素降解中起重要作用。关键词高温胁迫；多肉植物；花青素代谢中图分类号TS201.3文献标识码A文章编号1007-7731（2023）03-0029-05EffectsofHighTemperatureontheMetabolismofAnthocyaninsinAeoniumarboreum′Halloween′XUEYaSHIZhuyueLIShuminWANGTingCHENShutingZHAORongLISuhuaZHANGLihua（SuqianCollege,SuqianJiangsu223800）AbstractInordertostudytheeffectofhightemperaturestressontheanthocyaninmetabolismofAeoniumarboreum′Halloween′,thecuttingsof′Halloween′wereusedasmaterialsandtreatedat37℃for0h,6h,12h,24hand48h,respectively.Then,theanthocyanincontent,peroxidaseactivity,polyphenoloxidaseactivity,peroxidasegeneandpolyphenoloxidasegeneexpressionlevelsintheleavesof′Halloween′undereachtreatmentweredetermined.Theresultsshowedthattheanthocyanincontentintheleavesof′Halloween′showedadownwardtrendinthe48hofhightemperaturestresstreatment,buttherewasnosignificantchangeinthefirst12h;theactivityofpolyphenoloxidaseandperoxidaseintheleavesof′Halloween′showedatrendofincreasingfirstandthendecreasing,andbothreachedth...
