2023年河北大学学报(自然科学版)2023第43卷第2期JournalofHebeiUniversity(NaturalScienceEdition)Vol.43No.2DOI:10.3969/j.issn.10001565.2023.02.007甘露糖醛酸寡糖增敏环丙沙星抑制大肠埃希菌机制的研究谢笑莹1,郭怀忠1,2,曹丽军3,马梦楠1,崔秀彦4(1.河北大学药学院,河北保定071002;2.河北省药物质量分析控制重点实验室,河北保定071002;3.河北大学附属医院检验科,河北保定071000;4.河北大学附属医院药学部,河北保定071000)摘要:通过考察大肠埃希菌(Escherichia.coli)体内环丙沙星(ciprofloxacin,CPFX)的含量以及联用不同浓度甘露糖醛酸寡糖(MOS)对CPFX抑制E.coli效果的影响,探讨了MOS增敏CPFX抑制E.coli活性的作用机制.在菌液中加入CPFX和不同质量浓度的MOS,37℃培养150min,观察抑菌效果,并用HPLC法测定E.coli体内CPFX的含量,同时通过激光扫描共聚焦显微镜(CLSM)观察E.coli体内CPFX的荧光强度进行验证.还考察了Ca2+对MOS增敏CPFX抑菌效果的影响.结果显示:CPFX与高浓度的MOS联用时,E.coli体内CPFX的含量较低;与较低浓度的MOS联用时,E.coli体内CPFX的含量较高,与其抑菌实验联用MOS高浓度时拮抗,低浓度时增敏的结果一致.Ca2+的加入,在一定程度上逆转了高浓度MOS与CPFX的拮抗作用.该研究可为新型抗菌增效剂的开发和抗耐药菌感染的临床应用提供参考.关键词:甘露糖醛酸寡糖;环丙沙星;大肠埃希菌;Ca2+中图分类号:R917文献标志码:A文章编号:10001565(2023)02015607StudyonthemechanismofmannuronicacidoligosaccharidesensitizingciprofloxacinagainstEscherichiacoliXIEXiaoying1,GUOHuaizhong1,2,CAOLijun3,MAMengnan1,CUIXiuyan4(1.CollegeofPharmaceuticalSciences,HebeiUniversity,Baoding071002,China;2.KeyLaboratoryofPharmaceuticalQualityAnalysisandControlofHebeiProvince,Baoding071002,China;3.DepartmentofClinicalLaboratory,AffiliatedHospitalofHebeiUniversity,Baoding071000,China;4.DepartmentofPharmacy,AffiliatedHospitalofHebeiUniversity,Baoding071000,China)Abstract:Byinvestigatingthecontentofciprofloxacin(CPFX)inEscherichiacoliandtheeffectofmannuronicacidoligosaccharide(MOS)withdifferentconcentrationsontheinhibitoryactivityofCPFXagainstE.coli,themechanismthatMOSsensitizetheinhibitoryactivityofCPFXagainstE.coliwasexplored.CPFXandMOSwithdifferentconcentrationswereaddedintothebacterialsuspensi...
