18种观赏植物F3'5'H基因生物信息学分析曾慧兰1，2，高阳1，卢毅1(1．宜春学院生命科学与资源环境学院，江西宜春336000;2．江西省作物生长发育与调控重点实验室，江西宜春336000)摘要采用生物信息学方法对18种观赏植物类黄酮－3'5'－羟化酶基因(flavonoid－3'5'－hydroxylase，F3'5'H)的mＲNA和氨基酸序列的理化性质、跨膜结构域、保守结构域、亚细胞定位、二级结构、三级结构和同源性进行预测与分析。结果表明，绝大多数观赏植物的F3'5'H为亲水性稳定蛋白质，以α螺旋为主、无信号肽的跨膜蛋白质;大多数定位于内质网膜上;其三级结构模型为5ylw．1．A铁锈醇合成酶，为单链蛋白，属于细胞色素P450基因家族;同源保守氨基酸序列为“LPPGP”“AGTDTS”和“PFGAGＲＲICAG”。关键词生物信息学;观赏植物;氨基酸序列;类黄酮－3'5'－羟化酶中图分类号S68文献标识码A文章编号0517－6611(2023)07－0103－07doi:10．3969/j．issn．0517－6611．2023．07．026开放科学(资源服务)标识码(OSID):BioinformaticalAnalysisofFlavonoid-3'5'-HydroxylaseGenesOriginatedfrom18OrnamentalPlantsZENGHui-lan1，2，GAOYang1，LUYi1(1．CollegeofLifeScienceandＲesourcesandEnvironment，YichunUniversity，Yichun，Jiangxi336000;2．KeyLaboratoryofCropGrowth，DevelopmentandＲegulation，YichunUniversity，Yichun，Jiangxi336000)AbstractPredictiveanalysesofthemＲNAsequenceoraminoacidsequencesoftheflavonoid3'5'-hydroxylasegene(F3'5'H)originatedfrom18ornamentalplantsandtheirphysicalandchemicalparameters，transmembranedomains，conserveddomains，subcellularlocalizations，secondarystructure，tertiarystructureandevolutionaryrelationshipswereexploredusingbioinformaticsmethod．TheresultssuggestedthatmostF3'5'Hofthe18ornamentalplantsarehydrophilicstableproteinsandtransmembraneproteins，whicharemainlymadeofalpha-helixandnosignalpeptides;theyaremostlylocatedonendoplasmicreticulummembraneandassignedto5ylw．1．A，whichbelongstocytochromeP450su-perfamilyandisamonomernamedferruginolsynthaseintertiarystructureanalysis;theHomologousconservedaminoacidsequencesare“LPPGP”“AGTDTS”and“PFGAGＲＲICAG”．KeywordsBioinformatical;Ornamentalplants;Aminoacidsequence;Flavonoid-3'5'-hydroxylase基金项目江西省...
