农业环境科学学报JournalofAgro⁃EnvironmentScience2022,41(12):2752-27622022年12月沙月霞,黄泽阳,李云翔,等.生物菌剂对土壤微生物群落结构和功能的影响[J].农业环境科学学报,2022,41(12):2752-2762.SHAYX,HUANGZY,LIYX,etal.Impactofmicrobialagentsonthestructureandfunctionofthesoilmicrobialcommunity[J].JournalofAgro-EnvironmentScience,2022,41(12):2752-2762.开放科学OSID生物菌剂对土壤微生物群落结构和功能的影响沙月霞,黄泽阳,李云翔,赵沛(宁夏农林科学院植物保护研究所,银川750011)ImpactofmicrobialagentsonthestructureandfunctionofthesoilmicrobialcommunitySHAYuexia,HUANGZeyang,LIYunxiang,ZHAOPei(InstituteofPlantProtection,NingxiaAcademyofAgricultureandForestrySciences,Yinchuan750011,China)Abstract:Thisstudyexploredtheimpactofmicrobialagentsonthestructureofthemicrobialcommunityandfunctionalgenesinmaizefarmland.Themetagenomiclibrarywasusedtoanalyzethesoilmicrobialcommunitystructure,geneabundanceofnitrifyingfunctionalmicroorganisms,functionalannotationanddiversityofcarbohydrate-activeenzymes(CAZy)andantibioticresistantgenes(ARGs).TheresultsindicatedthattheabundanceofsoilmicroorganismsProteobacteria,Actinobacteria,andFirmicutesincreasedaftersoakingtheseedinWugufengsuandcombiningitwithmicrobialagents.Inaddition,theabundanceofAcidobacteriotia,Gemmatimonadetes,Chloroflexi,andCandidatusRokubacteriaalsodecreased.TheabundanceofThaumarchaeotaandEuryarchaeotainarchaeaincreasedby26.41%and9.09%,andtheabundanceofAscomycota,BasidiomycotaandChytridiomycotainfungidecreased.Asforsoilmicrobialgenus,therelative收稿日期:2022-10-18录用日期:2022-11-15作者简介:沙月霞(1974—),女,宁夏平罗人,博士研究生,从事植物病害生物防治及微生态学研究。E-mail:yuexiasha@126.com基金项目:宁夏重点研发计划项目(2022BBF02031);国家重点研发计划项目(2017YFD0201606);宁夏青年拔尖人才培养工程Projectsupported:TheKeyResearchandDevelopmentProgramofNingxia,China(2022BBF02031);TheNationalKeyResearchandDevelopmentProgramofChina(2017YFD0201606);TheYoungTop-notchTalentTrainingProjectofNingxia,China摘...