中国国境卫生检疫杂志2023年2月第46卷第1期ChineseFrontierHealthQuarantineFeb.2023，Vol46，No.1新发和再发病媒传播疾病（vector-bornedis-eases，VBDs）是全世界的公共卫生问题之一[1]。病媒生物可在人与人及动物与人之间传播寄生虫、病毒和细菌等病原体，引发疾病，如寨卡病毒病、登革热、黄热病等。全球化、城市化的发展加速了病媒生物随人员、货物或交通工具在地域间的迁移，加大了病媒生物的活动区域和传播疾病的范围。蚊类和蝇类是病媒传播疾病的重要传播媒介，对蚊类和蝇类进MolecularidentificationofmosquitoesandfliescapturedatQingdaoJiaodongInternationalAirportbeforeoperationLIUDi*，ZHUANGGuodong，XULi，YUYingquan，ZHANGTao，XUYing，TENGXindong*QingdaoCustomsDistrict，Qingdao，Shandong266071，ChinaAbstract：ObjectiveTounderstandthepopulationofmosquitoesandfliesinQingdaoJiaodongInternationalAirportbeforeoporation，andprovidedatasupportforthepreventionandcontrolofmedicalvectorsandvector-bornediseases.MethodsFromJulytoSeptember2019，fourareasincludingtheairportlawn，artificiallake，apronanddormitoryareaofJiaodongAirportportwereselected.Thedouble-layerednetmethodcombinedandcarbondioxidemosquitotrapswereusedtocaptureadultmosquitoes，theflycageswereusedtocaptureflies，andthescoopswereusedtocapturemosquitolarvaeandincubatethemnaturally.ThecapturedmosquitoesandflieswereclassifiedandidentifiedbyusingmorphologicalidentificationandmolecularidentificationmethodsbasedonmitochondrialCOIgene.ResultsFromJulytoSeptember2019，651mosquitoes（includingnaturallyhatchedmosquitoes）and2599flieswerecaptured，respectively.Combinedwithmorphologicalidentificationandmolecularidentification，itwasfinallydeterminedthattherewere6speciesofmosquitoesin4genera，1family.ThedominantspecieswereAnophelessinensis，CulexpipienspallensandAedesalbopictus，withtheconstituentrateof32.26%，27.65%and25.96%.Therewere13speciesoffliesbelongingto12generaand4families，thedominantspecieswereLuciliasericata，ChrysomyamegacephalaandLuciliacuprina，withtheconstituentrateof31.67%，28.51%and21.51%.AfteramplifyingthemitochondrialCOIgeneofmosquitoes...
