2023年第4期广东化工第50卷总第486期www.gdchem.com·17·鲁氏酵母高盐环境下的高密度发酵条件研究刘慧平1,2*，伍晓伟1,3，邓毛程1,2，李静1,2，叶茂1,2(1．广东轻工职业技术学院食品与生物技术学院，广东广州510300；2．广东省特色调味品工程技术开发中心，广东广州510300；3．广东石油化工学院生物与食品工程学院，广东茂名525000)[摘要]鲁氏酵母(Zygosaccharomycesrouxii)是一种广泛应用于酱油及酱类食品生产的耐高盐酵母。本研究采用单因素试验和正交试验对鲁氏酵母在高盐浓度下的高密度发酵培养基组成及培养条件进行优化。结果表明：鲁氏酵母的最优培养条件为：培养温度28℃，初始pH值5.5；最优培养基组合为葡萄糖10%，酵母粉2%，YNB2%，NaCl8%。在此优化条件下，鲁氏酵母的OD600nm值达到30.7，比优化前显著提高。该研究为高密度发酵获得耐盐高活性鲁氏酵母奠定基础。[关键词]鲁氏酵母；高盐；高密度发酵；发酵条件[中图分类号]TQ[文献标识码]A[文章编号]1007-1865(2023)04-0017-03StudyonHigh-DensityFermentationConditionsunderHigh-SaltEnvironmentofZygosaccharomycesRouxiiLiuHuiping1,2*,WuXiaowei1,3,DengMaocheng1,2,LiJing1,2,YeMao1,2(1.CollegeofFoodandBiologicalEngineering,GuangdongIndustryTechnicalCollege,Guangzhou510300；2.CentreofGuangdongforEngineeringandTechnologicalDevelopmentofSpecialtyCondiments,Guangzhou510300；3.CollegeofBiologyandFoodEngineering,GuangdongUniversityofPetrochemicalTechnology,Maoming525000,China)Abstract:Zygosaccharomycesrouxii，asanimprotantsalttolerantyeast,iswidelyappliedintheproductionofsoysauceandothersauces.Inthisstudy,theculturecompositionandfermentationconditionsforhigh-densityfermentationunderhigh-saltenvironmentwereoptimizedthroughsinglefactortestandorthogonaltest.TheresultsshowedthattheoptimumfermentationconditionsforZygosaccharomycesrouxiihigh-densityfermentationwereasfollows:fermentationtemperature30℃,initialpH5.5,theoptimalmediumcompositionwereglucose10%,yeastextract2%,YNB2%andNaCl8%.Undertheseoptimizedconditions,theOD600nmvalueofZygosaccharomycesrouxiiachievedto30.7,whichwassignificantlyhigherthanthatbeforeoptimization.Resultspresentedinthisstudymaycontributetoobtainsalt-tolerantandhigh-activi...
