402023年第1期（总第165期）·分析与应用·苦皮藤薄层色谱鉴别方法的建立罗健菱，赵婷婷，胡家震，唐娟，胡成刚*，马彬焰，邰通芝（贵州中医药大学，贵州贵阳，550025）摘要：为建立苦皮藤二氯甲烷部位的薄层色谱鉴别方法，该研究以雷公藤醌F（TriptoquinoneF）和Epikatonicacid为对照品,考察供试品溶液制备方法、展开剂、点样量、展距、显色剂对薄层色谱分析的影响,确定苦皮藤的最佳薄层色谱条件。结果显示：采用薄层色谱GF254硅胶板，以二氯甲烷为萃取溶剂制备供试品溶液，甲苯-乙酸乙酯-冰醋酸=7：2：0.2为展开剂展开，喷以5%磷钼酸乙醇溶液，热风吹干显色，于日光底部灯下进行检视，所得苦皮藤层色谱分离效果和显色效果较佳，斑点清晰且特征性好。该方法安全、易操作、专属性强,可作为苦皮藤二氯甲烷部位的薄层鉴别方法。关键词：薄层色谱鉴别；苦皮藤；展开剂；显色剂；中图分类号：R284.1文献标志码：A文章编号：1008-3103（2023）01-0040-05EstablishmentofTLCIdentificationMethodforCelastrusAngulatusMaximLuoJian-ling,ZhaoTing-ting,HuJia-zhen,TangJuan,HuCheng-gang*,MaBin-yan,TaiTong-zhi（SchoolofPharmacy,GuizhouUniversityofTraditionnalChineseMedicine,GuiyangGuizhou5500025,China）Abstract：InordertoestablishaTLCidentificationmethodforthedichloromethanepartofCelastrusangulatus,TripoquinoneFandEpikatonicacidwereusedasreferencesubstances,andtheeffectsofpreparationmethodoftestsolution,developingagent,sampleamount,spreadingdistanceandcolordeveloperonTLCanalysiswereinvestigated,andthebestTLCconditionsofCelastrusangulatusweredetermined.Theresultsshowedthatthin-layerchromatographyGF254silicagelplatewasusedtopreparethetestsolutionwithdichloromethaneasextractionsolvent,toluene-ethylacetate-glacialaceticacid=7：2：0.2asdevelopingagent,sprayedwith5%phosphomolybdicacidethanolsolution,driedbyhotairforcolordevelopment,andexaminedunderthesunlightbottomlamp.TheobtainedCelastrusangulatuslayerchromatographyhasgoodseparationandcolordevelopmenteffect,clearspotsandgoodcharacteristics.Themethodissafe,easytooperateandspecific,andcanbeusedasaTLCidentificationmethodfordichloromethanepartsofCelastrusangulatus.Keywords：TLCidentification；Bittervine；Develo...
