2023年第4期广东化工第50卷总第486期www.gdchem.com·197·基于UPLC指纹图谱和含量测定法评价不同干燥温度对羌活药渣质量的影响林伟雄，陈仕妍，黄森，刘权震，罗毓，吴晓英，刘燎原*，胡懿(广东一方制药有限公司，广东省中药配方颗粒企业重点实验室，广东佛山528244)[摘要]目的：建立UPLC指纹图谱和含量测定方法，评价不同干燥温度下羌活水提药渣的质量。方法：测定羌活药渣UPLC图谱以计算各共有峰的残存量，并结合聚类分析(CA)和主成分分析(PCA)优选最佳处理温度。建立含量测定方法以同时测得不同样品中羌活醇和异欧前胡素的含量。结果：CA和PCA得到相似的结论，将样品划分为三类。PCA综合评分表明60℃为样品的最适烘干条件，与含量测定结果一致。结论：本研究提供了稳定且具有专属性的指纹图谱和含量测定方法，并作为羌活药渣质量评价和后续开发的参考基础。[关键词]羌活；药渣；UPLC指纹图谱；含量测定；聚类分析；主成分分析[中图分类号]TQ[文献标识码]A[文章编号]1007-1865(2023)04-0197-06EffectofdryingtemperatureonQualityofNotopterygiiRhizomeResiduesbyUPLCFingerprintandComponentDeterminationLinWeixiong,ChenShiyan,HuangSen,LiuQuanzhen,LuoYu,WuXiaoying,LiuLiaoyuan*,HuYi(GuangdongYifangPharmaceuticalCo.,Ltd.,GuangdongProvincialKeyLaboratoryofTraditionalChineseMedicineFormulaGranule,Foshan528244,China)Abstract:Objective:ToestablishtheUPLCfingerprintchromatogramandquantificationmethodforwater-extractedresiduesformNotopterygiiRhizome,andevaluateitsqualitybydifferentdryingtemperatures.Methods:UPLCcharacterizationwasperformedonNotopterygiumresiduessothattocalculateremainingquantitiesofthecommonpeaks,onwhichclusteranalysis(CA)andprincipalcomponentanalysis(PCA)werecarriedouttoselecttheoptimalprocessingtemperature.Meanwhile,contentdeterminationmethodsweresetupforquantitycalculationofnotopterolandisoimperatorinsimultaneouslyamongvarioussamples.Results:ResultsofCAandPCAsharedthesimilarconclusion,inwhichallsampleswereclassifiedintothreegroups.Moreover,comprehensivescoringbyPCAsuggestedthat60℃wouldbetheoptimaldryingconditionforthesamples,whichwasconsistentwithoutcomesfromcontentanalysis.Conclusion:Theresearchprovidedboththestableandspecifiedfi...
