中国现代医学杂志ChinaJournalofModernMedicineVol.33No.7Apr.2023第33卷第7期2023年4月癌胚抗原相关细胞黏附分子源性多肽KM17对血小板活化作用的研究*万雯，王华炜，叶雨佳，李龙君，杨理宏，杨晓娜，董玲，陈丽星，孟照辉（昆明医科大学第一附属医院心内科，云南昆明650032）摘要：目的探讨癌胚抗原相关细胞黏附分子（CEACAM1）源性多肽KM17对血小板聚集、释放、黏附功能的影响。方法采用血小板聚集仪观察多肽KM17对凝血酶、胶原、花生四烯酸（AA）、二磷酸腺苷（ADP）等激动剂诱导的血小板聚集的影响；流式细胞术观察多肽KM17对ADP激活血小板后P-选择素释放的影响；显微镜下观察多肽KM17对血小板静态黏附于胶原基质的影响。结果多肽KM17能显著促进ADP诱导的血小板聚集且呈剂量依赖（P<0.05），而对AA、胶原及凝血酶诱导的血小板聚集差异均无统计学意义（P>0.05）；此外，多肽KM17对ADP激活血小板后P-选择素释放及血小板静态黏附于胶原基质的差异也无统计学意义（P>0.05）。结论多肽KM17可明显促进ADP诱导的血小板聚集，但对ADP活化血小板后P-选择素释放及血小板静态黏附于胶原基质未见明显作用。关键词：多肽KM17；血小板聚集；二磷酸腺苷；P-选择素；黏附中图分类号：R54文献标识码：AEffectofCEACAM1-derivedpeptideKM17onplateletactivation*WanWen,WangHua-wei,YeYu-jia,LiLong-jun,YangLi-hong,YangXiao-na,DongLing,ChenLi-xing,MengZhao-hui(DepartmentofCardiology,TheFirstAffiliatedHospitalofKunmingMedicalUniversity,Kunming,Yunnan650032,China)Abstract:ObjectiveToexploretheeffectofKM17,asyntheticCEACAM1-derivedpeptide,onplateletaggregation,release,andadhesion.MethodsWeexaminedtheeffectofKM17onplateletaggregationinducedbydifferentagonists(ADP,thrombin,collagen,andAA)withaggregometer.WeobservedtheeffectofKM17onplateletP-selectinreleaseafterstimulatedbyADPwithFCM.TheeffectofKM17onplateletadhesiontocollagenwasobservedunderamicroscope.ResultsKM17dose-dependentlypromotedADP-inducedplateletaggregation(P<0.05),buthadnosignificanteffectonplateletaggregationinducedbyAA,collagen,andthrombin(P>0.05).Inaddition,KM17hadnosignificanteffectonplateletP-selectinreleaseinducedbyADP(P>0.05),andtherewasnosignificantdifferenceinthenumbersofplateletadhesiontocollagenafterincubatedwi...
