1例ANA免疫荧光阴性而确诊干燥综合征的病例分析胡美娜,张云,马利莎,马德清(阿勒泰地区疾病预防控制中心,新疆阿勒泰836000)DOI:10.11748/bjmy.issn.1006-1703.2022.11.023收稿日期:2022-01-11;修回日期:2022-09-16作者简介:胡美娜,女,副主任技师,主要从事临床检验研究。E-mail:1252563388@qq.com摘要:目的通过1例ANA免疫荧光阴性而确诊干燥综合征的病例研究,分析抗核抗体不同方法学试剂检测结果存在差异的原因,探讨自身抗体不同方法学试剂搭配使用策略以及广谱筛查在自身免疫病早期诊断中的重要性。方法对1例确诊的干燥综合征病例进行不同方法学抗核抗体检测以及不同类型自身抗体谱筛查。结果患者女,45岁,眼干、口干、多年前确诊为干燥综合征。ANA免疫荧光结果为阴性,化学发光结果为阳性,进一步筛查发现抗SS-A/Ro抗体阳性、抗环瓜氨酸肽抗体阳性、类风湿因子阳性。同时患者存在晨僵与关节肿胀,完全符合类风湿关节炎早期诊断标准,确诊为干燥综合征合并类风湿关节炎。结论自身抗体检测应注重多种方法学的合理搭配,同时对于临床高度疑似自身免疫病的患者,高危人群建议进行自身抗体广谱筛查,有助于潜在自身免疫病的早期诊断和早期治疗。关键词:干燥综合征;抗核抗体;间接免疫荧光;化学发光免疫分析;早期诊断中图分类号:R593.2文献标识码:AAntinuclearAntibody-NegativeSjögren’sSyndromeUsingIndirectImmunofluorescence:aCaseAnalysisHUMeina,ZHANGYun,MALisha,MADeqing(CenterforDiseaseControlandPrevention(CDC),Aletai836000,China)Abstract:ObjectiveByanalyzingacaseofANAimmunofluorescence-negativeSjögren’sSyndrome,toexplorereasonsleadingtodifferentresultsofdifferentmethodologicalreagentsforANA,andtoexploretheuseofdifferentmethodologicalreagentsforautoantibodiesandtheimportanceofbroad-spectrumscreeningintheearlydiagnosisofautoimmunediseases.MethodsAconfirmedcaseofSjögren’sSyndromewastestedwithdifferentmethodologiesforantinuclearantibodyanddifferenttypesofautoantibodyprofilescreening.ResultsA45-year-oldfemalepresentedwithoralandocularsymptoms,diagnosedwithSjögren’sSyndromemanyyearsagowasstudied.HerANAwasnegativebyIIF,whilepositivebyCLIA.Furtherscreeningrevealedpositiveanti-SS-A/Roantibodies,positiveanti-cycliccitrullinatedpeptideantibodies,andpositiverheumatoidfactor.This...
