Designation:D4199−82(Reapproved2011)StandardTestMethodsforAutoclavabilityofMembraneFilters1ThisstandardisissuedunderthefixeddesignationD4199;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thesetestmethodsdealwithteststodeterminetheautoclavabilityofmembranefilters.1.2Thesetestmethodsareapplicabletoallmembranefilters.EitherTestMethodA,TestMethodB,orboth,maybeutilizedtoassesstheautoclavabilityofmembranefilters.SectionTestMethodA—DimensionalStability8.1TestMethodB—WettingCharacteristics8.21.3Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafetyandhealthpracticesanddeterminetheapplica-bilityofregulatorylimitationspriortouse.2.ReferencedDocuments2.1ASTMStandards:2D1129TerminologyRelatingtoWaterD1193SpecificationforReagentWater3.Terminology3.1Definitions—Fordefinitionsoftermsusedinthesetestmethods,refertoTerminologyD1129.4.SummaryofTestMethods4.1TestMethodA—Autoclavabilityisevaluatedbymeasur-ingthediameterofthemembranefilter,acrossthreeaxes,priortoautoclavingandafterautoclaving,todeterminedimensionalstability.4.2TestMethodB—Wettabilityisdeterminedbyplacingsamplesofautoclavedandnonautoclavedmembranefiltersinwaterandmeasuringthetimerequiredforthefilterstobecomecompletelywet.5.SignificanceandUse5.1Thesetestmethodsprovideinformationonthedimen-sionalstabilityandwettingcharacteristicsofautoclavedmem-branefilters.Excessivedimensionalchangescausedbyauto-clavingaconstrainedmembranefiltercouldcrackthefilter,renderingituseless.Increasedhydrophobicity,duetoautoclav-ingwouldresultinanincreasedtimetofilteraliquidsample.6.Apparatus6.1Calipers,accurateto0.1mm.6.2Holder,suitableformembranefilters.6.3Autoclave.6.4PetriDish.6.5Forceps,nonserrated.6.6Stopwatch,accu...
