Designation:D3048−89(Reapproved2016)StandardTestMethodofAssayforAlkalineProtease1ThisstandardisissuedunderthefixeddesignationD3048;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thistestmethodcoverstheassayofalkalineproteaseenzymes.Thisprocedureisapplicabletoenzymepreparationswithhighactivitybutisinapplicabletoformulateddetergentproductsorairsamples.1.2ThevaluesstatedinSIunitsaretoberegardedasstandard.Nootherunitsofmeasurementareincludedinthisstandard.1.3Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafetyandhealthpracticesanddeterminetheapplica-bilityofregulatorylimitationspriortouse.MaterialSafetyDataSheetsareavailableforreagentsandmaterials.Reviewthemforhazardspriortousage.2.ReferencedDocuments2.1ASTMStandards:2D459TerminologyRelatingtoSoapsandOtherDetergentsD1129TerminologyRelatingtoWaterD1193SpecificationforReagentWaterE131TerminologyRelatingtoMolecularSpectroscopy3.Terminology3.1Definitions:3.1.1APBunit—thatamountofenzymewhichreleasesin1minundertheconditionsofthetestacaseinhydrolysatethathasthesameabsorbanceas1µgoftyrosineinanequivalentvolume.ThenumberofAPBunitspergramofapreparationiscalledtheAPBofthepreparation.3.1.2standardizedenzyme—anenzymepreparationofknownactivityforcalibratingthesampleenzymeintermsofagravimetricstandardofenzymaticactivity.3,43.1.3Theterms“alkylbenzenesulfonate(ABS)”and“lin-earalkylatesulfonate(LAS)”inthismethodaredefinedinaccordancewithTerminologiesD1129andD459:3.1.3.1alkylbenzenesulfonate(ABS)—thegenericnameappliedtotheneutralizedproductresultingfromthesulfona-tionofanalkylatedbenzene.3.1.3.2linearalkylatesulfonate(LAS)—aformofalkylbenzenesulfonate(ABS)inwhichthealkylgroupislinearratherthanabranchedchai...
