Designation:D4576−08(Reapproved2013)StandardTestMethodforMoldGrowthResistanceofWetBlue1ThisstandardisissuedunderthefixeddesignationD4576;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thistestmethodcoversthedeterminationofmoldgrowthresistanceofwetblueandwetwhitesubjecttostorageandshippingrequirementsandintendedforuseinleathermanufacturing.Thistestmethodmaynotbesuitabletoevaluatefungicidesthatareinactivatedbyproteins.Thisincludesalkyldimethylbenzylammoniumchlorides.1.2Conclusionsaboutmoldgrowthresistancearedrawnfromtheresultsbycomparingthetestwithasimultaneouslyruncontrolofknownresistance.Successorfailureisdeter-minedbytheamountofmoldgrowthrelativetothecontrol.1.3Toallowuseofthistestmethodbyanylaboratory,flexibilityhasbeenpermittedintimes,temperature,andhumidityofincubation,inoculum,hidesamplingarea,andchoiceofcontrol.Thesemaybeadjustedtofitlocalconditionsbutmustbestandardized.1.4Formoldgrowthresistanceofwetwhite,theprocedureisidentical,substitutewetwhiteforwetblueinthestandardmethod.1.5ThevaluesstatedinSIunitsaretoberegardedasstandard.Nootherunitsofmeasurementareincludedinthisstandard.1.6Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafetyandhealthpracticesanddeterminetheapplica-bilityofregulatorylimitationspriortouse.2.Terminology2.1DefinitionsofTermsSpecifictoThisStandard:2.1.1wetblue—hideorskin,orsplitofahideorskin,tannedwithbasicchromiumsulfate,containingapproximately50%moistureandhavinganacidicpH.2.1.2wetwhite—ahideorskin,orsplitofahideorskintannedwithorganicornon-organictanningagents(excludingchromiumorironcontainingagentsandvegetableextracts),containingapproximately50%moisture.3.SummaryofTestMethod3.1Wetbluetestspecimensaresurrounded...