Designation:D5392−14StandardTestMethodforIsolationandEnumerationofEscherichiaColiinWaterbytheTwo-StepMembraneFilterProcedure1ThisstandardisissuedunderthefixeddesignationD5392;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thistestmethoddescribesamembranefilter(MF)procedureforthedetectionandenumerationofEscherichiacoli,abacteriumfoundexclusivelyinthefecesofhumansandotherwarm-bloodedanimals.Thepresenceofthesemicroor-ganismsinwaterisanindicationoffecalpollutionandthepossiblepresenceofentericpathogens.Thesebacteriaarefoundinwaterandwastewaterinawiderangeofdensities.Thedetectionlimitofthisprocedureisonecolonyformingunit(CFU)pervolumefiltered.1.2Thistestmethodhasbeenusedsuccessfullywithtemperatefreshandmarineambientwaters,andwastewaters.Itistheuser’sresponsibilitytoensurethevalidityofthistestmethodforwatersofothertypes.1.3ThevaluesstatedinSIunitsaretoberegardedasstandard.1.4Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafetyandhealthpracticesanddeterminetheapplica-bilityofregulatorylimitationspriortouse.Forspecifichazardstatements,seeSection9.2.ReferencedDocuments2.1ASTMStandards:2D1129TerminologyRelatingtoWaterD1193SpecificationforReagentWaterD3370PracticesforSamplingWaterfromClosedConduitsD3870PracticeforEstablishingPerformanceCharacteristicsforColonyCountingMethodsinMicrobiology(With-drawn2000)3D5465PracticeforDeterminingMicrobialColonyCountsfromWatersAnalyzedbyPlatingMethods3.Terminology3.1Definitions:3.1.1Fordefinitionsoftermsusedinthistestmethod,refertoTerminologyD1129.3.2DefinitionsofTermsSpecifictoThisStandard:3.2.1Escherichiacoli(E.coli),n—aspeciesofbacteriathatisamemberofthetotalcoliformgroupandknowntooriginateinthefecesofwarm-bloodeda...
