Designation:D5712−15(Reapproved2020)StandardTestMethodforAnalysisofAqueousExtractableProteininLatex,NaturalRubber,andElastomericProductsUsingtheModifiedLowryMethod1ThisstandardisissuedunderthefixeddesignationD5712;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thistestmethodcoversananalyticaltestfordetermin-ingtheamountoftotalaqueousextractableproteinassociatedwithNR,latex,andelastomericproducts.Watersolubleproteinsareextractedinabuffersolutionandthenprecipitatedtoconcentratethemandalsotoseparatethemfromwatersolublesubstancesthatmayinterferewiththedetermination.Theextractedproteinisredissolvedandquantifiedcolorimetri-callybythemodifiedLowrymethodusingaproteinstandard.1.2Forthepurposeofthistestmethod,therangeofproteinmeasurementwillbebasedonthelimitofdetectionandquantitationandrecordedinmicrogramsperdm2testspeci-men.1.3Thetestmethodisdesignedtobeaccurateandcompat-iblewiththeindustrialenvironment.1.4Stepsareincludedinthistestmethodtominimizetheeffectsofinterferingsubstances.1.5Itisrecognizedthatothermethodsfortheanalysisofleachableproteinsexistandthesemaybeusedforroutinequalitycontrolpurposesprovidedtheyhavebeenvalidatedandacorrelationestablishedagainstthereferencemethodspecifiedbythistestmethod.1.6Thistestmethodhasnotbeenvalidatedforusewithlubricatedproductssuchascondoms.Condomswithdifferentlubricantsastypicallymarketed,havenotbeentestedinanASTMILStodetermineif,andifsotowhatdegree,thelubricantinterfereswiththeassay.1.7Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafety,health,andenvironmentalpracticesanddeter-minetheapplicabilityofregulatorylimitationspriortouse.1.8Thisinternationalstandardwasdevelopedinaccor-dancewithinternationallyrecognize...
