Designation:D5590−00(Reapproved2010)´1StandardTestMethodforDeterminingtheResistanceofPaintFilmsandRelatedCoatingstoFungalDefacementbyAcceleratedFour-WeekAgarPlateAssay1ThisstandardisissuedunderthefixeddesignationD5590;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.ε1NOTE—Aneditorialchangewasmadein7.5inNovember2012.1.Scope1.1Thistestmethodcoversanacceleratedmethodfordeterminingtherelativeresistanceoftwoormorepaintsorcoatingfilmstofungalgrowth.1.2ThevaluesstatedinSIunitsaretoberegardedasthestandard.Thevaluesgiveninparenthesesareforinformationonly.1.3Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafetyandhealthpracticesanddeterminetheapplica-bilityofregulatorylimitationspriortouse.2.ReferencedDocuments2.1ASTMStandards:2D822PracticeforFilteredOpen-FlameCarbon-ArcExpo-suresofPaintandRelatedCoatingsD3273TestMethodforResistancetoGrowthofMoldontheSurfaceofInteriorCoatingsinanEnvironmentalCham-berD3456PracticeforDeterminingbyExteriorExposureTeststheSusceptibilityofPaintFilmstoMicrobiologicalAttackD4141PracticeforConductingBlackBoxandSolarCon-centratingExposuresofCoatingsD4587PracticeforFluorescentUV-CondensationExpo-suresofPaintandRelatedCoatingsD5031PracticeforEnclosedCarbon-ArcExposureTestsofPaintandRelatedCoatingsG21PracticeforDeterminingResistanceofSyntheticPoly-mericMaterialstoFungi3.SummaryofTestMethod3.1Thistestmethodoutlinesaprocedureto(1)prepareasuitablespecimenfortesting,(2)inoculatethespecimenwiththeproperfungalspecies,(3)exposetheinoculatedsamplesundertheappropriateconditionsforgrowth,and(4)provideascheduleandguidelinesforvisualgrowthratings.Thistestmethodisnotdesignedtoincludeallthenecessaryprocedurestomaintainthepropermicrobiologicaltechniquesr...