Designation:D7817−12(Reapproved2021)StandardTestMethodforEnumerationofYeastandMoldinRacewayBrine,Brine-CuredHidesandSkins1ThisstandardisissuedunderthefixeddesignationD7817;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thistestmethodcoverstheenumerationofyeastandmold.Thistestmethodisapplicabletoracewaybrine,brine-curedhidesandskins,andpre-chargeracewayliquor.1.2ThevaluesstatedinSIunitsaretoberegardedasstandard.Nootherunitsofmeasurementareincludedinthisstandard.1.3Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafety,health,andenvironmentalpracticesanddeter-minetheapplicabilityofregulatorylimitationspriortouse.1.4Thisinternationalstandardwasdevelopedinaccor-dancewithinternationallyrecognizedprinciplesonstandard-izationestablishedintheDecisiononPrinciplesfortheDevelopmentofInternationalStandards,GuidesandRecom-mendationsissuedbytheWorldTradeOrganizationTechnicalBarrierstoTrade(TBT)Committee.2.ReferencedDocuments2.1ASTMStandards:2D6715PracticeforSamplingandPreparationofFreshorSalt-Preserved(Cured)HidesandSkinsforChemicalandPhysicalTestsE691PracticeforConductinganInterlaboratoryStudytoDeterminethePrecisionofaTestMethodE177PracticeforUseoftheTermsPrecisionandBiasinASTMTestMethods3.SummaryofTestMethod3.1Samplesofbrine-curedhidesandskins,racewaybrine,orpre-chargeracewayliquorareseriallydilutedandplatedonagarcontaining7%NaClandanantibioticsolution.Theplatesareincubatedat20–25°Cfor5days.4.SignificanceandUse4.1Thistestmethodenumeratessalttolerantyeastandmold,andundertheconditionsofthistestmethodthoseareequatedashalophilicorganisms.Salttolerantyeastandmoldhavebeenknowntocausedamagetohidesandskinsinracewaybrine.5.Apparatus5.1Incubator,20–25°C.5.2Colon...
