Designation:D7819−12(Reapproved2021)StandardTestMethodforEnumerationofYeastandMoldonFresh(Uncured)HidesandSkins1ThisstandardisissuedunderthefixeddesignationD7819;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thistestmethodcoverstheenumerationofyeastandmoldonfresh(uncured)hidesandskins.Thistestmethodisapplicabletouncuredhidesandskins.1.2ThevaluesstatedinSIunitsaretoberegardedasstandard.Nootherunitsofmeasurementareincludedinthisstandard.1.3Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafety,health,andenvironmentalpracticesanddeter-minetheapplicabilityofregulatorylimitationspriortouse.1.4Thisinternationalstandardwasdevelopedinaccor-dancewithinternationallyrecognizedprinciplesonstandard-izationestablishedintheDecisiononPrinciplesfortheDevelopmentofInternationalStandards,GuidesandRecom-mendationsissuedbytheWorldTradeOrganizationTechnicalBarrierstoTrade(TBT)Committee.2.ReferencedDocuments2.1ASTMStandards:2D6715PracticeforSamplingandPreparationofFreshorSalt-Preserved(Cured)HidesandSkinsforChemicalandPhysicalTestsE691PracticeforConductinganInterlaboratoryStudytoDeterminethePrecisionofaTestMethodE177PracticeforUseoftheTermsPrecisionandBiasinASTMTestMethods3.SummaryofTestMethod3.1Samplesofuncuredhidesandskinsareseriallydilutedandplatedonagarcontaininganantibioticsolution.Theplatesareincubatedat20–25°Cfor5days.4.SignificanceandUse4.1Thistestmethodenumeratesyeastandmold.Yeastandmoldhavebeenknowntocausedamagetohidesandskins.5.Apparatus5.1Incubator,20–25°C.5.2Colonycounter(notmandatory,buthighlyrecom-mended).5.3Sterilepipets.5.4Stomacher,formixinginitialdilution.(Ifstomacherisunavailable,hand-mix.)5.5Balance.5.6Sterilepetridishes.5.7Autoclave(sterili...
