Designation:E1415−91(Reapproved2012)StandardGuideforConductingStaticToxicityTestsWithLemnagibbaG31ThisstandardisissuedunderthefixeddesignationE1415;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thisguidedescribesproceduresforobtaininglaboratorydataconcerningtheadverseeffectsofatextmaterialaddedtogrowthmediumonacertainspeciesofduckweed(LemnagibbaG3)duringa7-dayexposureusingthestatictechnique.Theseprocedureswillprobablybeusefulforconductingtoxicitytestswithotherspeciesofduckweedandotherfloatingvascularplants,althoughmodificationsmightbenecessary.1.2Specialneedsorcircumstancesmightalsojustifymodi-ficationofthisstandard.Althoughusingappropriateproce-duresismoreimportantthanfollowingprescribedprocedures,resultsoftestsconductedusingunusualproceduresarenotlikelytobecomparabletoresultsofmanyothertests.Com-parisonofresultsobtainedusingmodifiedandunmodifiedversionsoftheseproceduresmightprovideusefulinformationconcerningnewconceptsandproceduresforconductingtestswithduckweed.1.3Theproceduresinthisguideareapplicabletomostchemicals,eitherindividuallyorinformulations,commercialproducts,orknownmixtures.WithappropriatemodificationstheseprocedurescanbeusedtoconducttestsontemperatureandpHandonsuchothermaterialsasaqueouseffluents(seealsoGuideE1192),leachates,oils,particulatematter,sedi-mentsandsurfacewaters.Theseproceduresdonotspecificallyaddresseffluentsbecausetodatethereislittleexperienceusingduckweedsineffluenttestingandsuchtestsmayposeproblemswithacclimationofthetestorganismstothereceivingwater.Statictestsmightnotbeapplicabletomaterialsthathaveahighoxygendemand,arehighlyvolatile,arerapidlybiologicallyorchemicallytransformedinaqueoussolution,orareremovedfromtestsolutionsinsubstantialquantitiesbythetestcham-bersororganismsduringthetest.1.4Resultsoftoxicitytestspe...
