Designation:E2315−03(Reapproved2008)StandardGuideforAssessmentofAntimicrobialActivityUsingaTime-KillProcedure1ThisstandardisissuedunderthefixeddesignationE2315;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thisguidecoversexamplesofabasicmethodtomeasurethechangesofapopulationofaerobicmicroorgan-ismswithinaspecifiedsamplingtimewhentestedagainstantimicrobialtestmaterialsinvitro.Severaloptionsfororgan-ismselectionandgrowth,inoculumpreparation,samplingtimesandtemperaturesareprovided.Whenthebasictechniqueisperformedasaspecifictestmethoditiscriticalwhenevaluatingtheresultstoensurethatsuchvariableshavebeenstandardized.Antimicrobialactivityofspecificmaterials,asmeasuredbythistechnique,mayvarysignificantlydependingonvariablesselected.Itisimportanttounderstandthelimita-tionsofinvitrotests,especiallycomparisonsofresultsfromtestsperformedunderdifferentcircumstances.Asanexample,testresultsofmicroorganismsrequiringgrowthsupplements,orspecialincubationconditions,maynotbedirectlycompa-rabletomorerobustorganismsundertheconditionsofasingleprocedure.1.2Knowledgeofmicrobiologicaltechniquesisrequiredforthistest.1.3ThevaluesstatedinSIunitsaretoberegardedasstandard.Nootherunitsofmeasurementareincludedinthisstandard.1.4Thisstandardmayinvolvehazardousmaterials,opera-tionsandequipment.Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatoryrequirementspriortouse.2.ReferencedDocuments2.1ASTMStandards:2D1193SpecificationforReagentWaterE1054TestMethodsforEvaluationofInactivatorsofAnti-microbialAgents3.Terminology3.1Definitions:3.1.1inoculumsuspension,n—theinitialsuspensionoftestorganismusedtoinoculatethetestmaterialTh...
