Designation:E2111−12StandardQuantitativeCarrierTestMethodtoEvaluatetheBactericidal,Fungicidal,Mycobactericidal,andSporicidalPotenciesofLiquidChemicals1ThisstandardisissuedunderthefixeddesignationE2111;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.INTRODUCTIONTheneedforbetterteststoassessthemicrobicidalactivityofchemicalswasrecognized(1)2andseveralsimplerandquantitativetestmethodshavebeendevelopedforworkingwithawidevarietyofmicroorganisms(2).Thetestmethoddescribedhereusesglassvialsascarriers;thesamebasicsetofmaterialsandprocedurescanbeusedtotestthepotencyofliquidmicrobicidesagainstvegetativebacteria,fungi,mycobacteria,andbacterialspores.However,thetestmethodisnotappropriateforusewithvirusesbecauseoftherelativelyhighlevelsofeluatedilutionsrequiredandtheneedformembranefiltration.Furtherevaluationofproductsundermorestringenttestconditionsmaybenecessaryfortheirregistration.Performancestandardsforthecategoriesofproductstobetestedandthespecifictypesoforganism(s)tobeusedmayalsovarydependingontheregulatoryagency.1.Scope1.1Thistestmethodisdesignedforuseinproductdevel-opmentandforthegenerationofproductpotencydata.Thistestmethodpermitstheloadingofeachcarrierwithaknownvolumeofthetestorganism.Theincorporationofcontrolscanalsodeterminetheinitialloadofcolonyformingunits(CFU)oforganismsonthetestcarriersandanylossinCFUafterthemandatorydryingoftheinoculum.1.2Thistestmethodisdesignedtohavesurvivorsandalsotobeusedwithaperformancestandard.Thesurvivingmicro-organismsoneachtestcarrierarecomparedtothemeanofnolessthanthreecontrolcarrierstodetermineiftheperformancestandardhasbeenmet.Toallowproperstatisticalevaluationofresults,thesizeofthetestinoculumshouldbesufficientlylargetotakeintoaccountboththeperformancestandardandtheexperimentalvariationintheresults.Forexample,ifanarbitrarype...
