Designation:E1147–92(Reapproved2005)StandardTestMethodforPartitionCoefficient(N-Octanol/Water)EstimationbyLiquidChromatography1ThisstandardisissuedunderthefixeddesignationE1147;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(e)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thistestmethoddescribesaprocedurefortheestima-tionofthelogoftheoctanol/waterpartitioncoefficient(logKow)ofchemicalsovertherangefrom0to8.1.2Thistestmethodusesanempiricallyderivedequationtorelatetheoctanol/waterpartitioncoefficienttoanexperimen-tallydeterminedretentiontimeonaliquidchromatographiccolumn.1.3ThistestmethodhasbeendesignedtoestimatelogKowvaluesforbothnon-ionizableandionizablecompounds.ThisisaccomplishedbybufferingtheliquidchromatographicsolventatapHthatwillforcethetestcompoundintoeitherthenon-ionizedorionizedform.1.4Thistestmethodrequiressomeknowledgeofthedetectorresponsetothechemicalbeingtested.1.5Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishsafetyandhealthpracticesanddeterminetheapplicabilityofregu-latorylimitationspriortouse.2.ReferencedDocuments2.1ASTMStandards:2D1193SpecificationforReagentWaterE200PracticeforPreparation,Standardization,andStor-ageofStandardSolutionsforChemicalAnalysisE682PracticeforLiquidChromatographyTermsandRe-lationshipsE1022PracticeforConductingBioconcentrationTestswithFishesandSaltwaterBivalveMolluscs3.Terminology3.1Definitions:3.1.1octanol/waterpartitioncoeffıcient(Kow)—theequilib-riumratioofthemolarconcentrationsofachemicalinn-octanolandwater,indilutesolution.Kowisaconstantforaspecificchemicalatagiventemperature.SinceKowistheratiooftwomolarconcentrations,itisadimensionlessquantity.KowisoftenreportedaslogKow.3.1.2retentiontime(tR,to)—thereferencecompoundortestchemicalretentiontime(tR)isthetimef...
