Designation:E1286–00StandardGuideforIdentificationofHerpesSimplexVirusorItsDNA1ThisstandardisissuedunderthefixeddesignationE1286;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(e)indicatesaneditorialchangesincethelastrevisionorreapproval.INTRODUCTIONThisguidecoverstheidentificationofherpessimplexvirus(HSV)oritsDNAandwasdevelopedbySubcommitteeE48.02onCharacterizationandIdentificationofBiologicalSystems.TheobjectiveistodescribelaboratorycharacterizationproceduresthatwouldbesufficienttoverifythatabiologicalpreparationbelievedtocontainprimarilyHSV(orHSVDNA)foruseinanystepofabiotechnologyprocessactuallydoescontainthisvirus(oritsDNA).Thisguideassumesabasicknowledgeofvirologyandmolecularbiology.1.Scope1.1Thisguidecoverslaboratorycharacterizationproce-duressufficienttoidentifypurifiedspecimensofHSVtypes1and2(HSV-1andHSV-2)orHSV-1DNAandHSV-2DNAusedinbiotechnology.ForcasesinwhichidentificationofHSVDNAspecimensisrequired,thecharacterizationcriteriaof6.2and6.3ofthisguidearesufficient.1.2ThisguidedoesnotcovertheidentificationofHSVinHSV-infectedhostcells.Toapplythisguidetosuchacase,itwouldfirstbenecessarytoisolatethevirusfromsuchsamplesusingstandardtechniquesofHSVpurification.ThisguidedoesnotcovercharacterizationofsegmentsofHSVDNAorofvectorscontainingHSVDNAsegments.1.3Thisguidedoesnotcoverthespecificmethodologyusedintheidentificationcharacterization.Itdoesnotaddressthequestionofdegreeofpurityrequiredforherpesvirusprepara-tions:thiswouldvarydependingontheparticularbiotechnol-ogyuseofthevirus.1.4Warning—Laboratoryworkinvolvingherpessimplexvirusescanbehazardoustopersonnel.Precaution:Biosafety2levelfacilitiesarerecommended(1).2SafetyguidelinesshallbeadheredtoaccordingtoNCCLSM29–T2andotherrecom-mendations(1).1.5Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstan...
